5岁以下儿童腹泻病例分离大肠埃希菌的PFGE基因分型研究

IF 0.8 Q3 MEDICINE, GENERAL & INTERNAL

Bangladesh Journal of Medical Science Pub Date : 2023-05-21 DOI:10.3329/bjms.v22i20.66302

Shahla Najim Abed Al Azzawi, R. Abdullah

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引用次数: 0

摘要

从5岁以下儿童腹泻病例中分离得到25株大肠埃希菌，具有广泛耐药(XDR)耐药模式，并对分离株进行敏感性筛选。E7 4组的抗生素有耐药性隔离和E5 - E13 E14灯头- E17 E18 - E20 E47 E46 - E42 E31 - E26隔离到5组抗生素产生抗药性和48 e隔离电阻组成的6组抗生素和E2 E38 - E36 E34 E28 - E16天E10isolates显示抗生素抵抗7组,和出价- E24 E19 E9 e1分离到8组抗生素的耐药性。采用限制性内切酶(Xbal)对25株大肠杆菌进行脉冲场凝胶电泳(PFGE)分型，分别为A组、B2组、D组(2/25和8%)、C组(4/25和16%)、F组(3/25和12%)和E组(12/25和48%)。分离株的遗传趋同率为83%，这是由于遗传内容、分离源和分离地本身具有相似性。《孟加拉国医学科学杂志》第22卷(特刊)2023年第18-24页

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Genotyping of Escherichia Coli Isolated from Diarrhea Cases in Children under Five Years of age Using the PFGE Method

Twenty –five isolates of Escherichia coli isolated from cases of diarrhea in children under five years of age were obtained, characterized by Extensively Drug-Resistant(XDR) resistance patterns, and sensitivity screening of isolates was performed.E7 isolates were resistant to 4 groups of antibiotics and E5 - E13 - E14 - E17 - E18 - E20 - E47 - E46 - E42 - E31 - E26 isolates resistant to 5 groups of antibiotics and 48E isolate resistance to 6 groups of antibiotics and consisting of E2 E38 - E36 - E34 - E28 - E16 - E10isolates showed resistance to 7 groups of antibiotics, and E41 - E24 - E19 - E9 –E1 isolates resistance to 8 groups of antibiotics. The genotyping of 25 E.coliisolates was carried out by Pulsed-field Gel Electrophoresis (PFGE) using restriction endonucleases (Xbal) which belong to different phylogenetic groups, were as group A, group B2, group D (2/25 and 8%) group C (4/25 and 16%), group F (3/25 and 12%), group E (12/25 and 48%). The genetic convergence rate of isolates is 83%, and this is due to the similarity in genetic content, the source of isolation, and the place of isolation itself. Bangladesh Journal of Medical Science Vol.22 (Special Issue) 2023 p.18-24

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