2013年新疆乌苏古尔特市波状黄疸及寄生蚤鼠疫耶尔森菌检测结果分析

Q3 Medicine

中华地方病学杂志 Pub Date : 2015-04-20 DOI:10.3760/CMA.J.ISSN.2095-4255.2015.04.012

Wang Xinhui, A. Bulikemuabudurexiti, S. Shi, A. Bulimitimaituohuti, L. Bing, A. Zhatirehemu, Re Natuerdi, Xu Bingchen

{"title":"2013年新疆乌苏古尔特市波状黄疸及寄生蚤鼠疫耶尔森菌检测结果分析","authors":"Wang Xinhui, A. Bulikemuabudurexiti, S. Shi, A. Bulimitimaituohuti, L. Bing, A. Zhatirehemu, Re Natuerdi, Xu Bingchen","doi":"10.3760/CMA.J.ISSN.2095-4255.2015.04.012","DOIUrl":null,"url":null,"abstract":"Objective \nTo observe immunological and bacteriological dynamic and distribution of Yersinia pestis in Citellus undulates in Gurt plague natural focus in Usu, Xinjiang in 2013. \n \n \nMethods \nIn the focus,Citellus undulates was captured with cram frame and blood from heart was collected and serum was isolated from June to August 2013. Indirect hemagglutination test (IHA) and enzyme-linked immunosorbent assay (ELISA) were taken to detect F1 antibody against Yersinia pestis of Citellus undulates, and the accuracy of two methods was conducted. Parasitic fleas were collected and classified. Yersinia pestis was isolated with four-step method (microscopic examination, cultivation for Yersinia pestis, bacteriovirus test and animal experiment) from liver, spleen of mice and parasitic fleas. \n \n \nResults \nTotally 436 Citellus undulates were captured and 2 801 parasitic fleas were collected. Positive rate of F1 antibody was 19.0% (83/436) and 4.4% (19/436) by ELISA and IHA, geometric mean titer (GMT) was 1∶26.92 and 1∶23.17, respectively. The positive rate and GMT of ELISA was higher than IHA (χ2 = 45.44,t = 1.96,P < 0.01 or < 0.05). The positive rate of F1 antibody was 20.2% (38/188), 25.9% (33/127) and 9.9% (12/121) from June, July to August, respectively, and the difference between months was statistically significant (χ2 = 10.67,P < 0.01). Totally 32 strains of Yersinia pestis were isolated. From June to August, the isolated strains were accounted for 81.3% (26/32), 12.5% (4/32) and 6.3% (2/32), respectively, the difference between months was statistically significant (χ2 = 20.60,P < 0.01). The ratio of strains isolated from 3 kinds of samples was 56.3% (18/32) of the fleas, 25.0% (8/32) of self-dead rodents and 18.8% (6/32) of the captured rodents, the difference between samples was statistically significant (χ2 = 18.46,P < 0.01). \n \n \nConclusions \nELISA is better than IHA for detecting sera F1 antibody, and testing accuracy can be improved by combination of the two methods. Strain detection rate is higher in June, and positive rate of self dead rodents is higher than that of captured rodents and fleas. More attention should be paid to strains isolation from the fleas in plague surveillance. Searching for self dead rodents is an effective way for strains isolation. \n \n \nKey words: \nCitellus undulates; Parasitic fleas; Yersinia pestis; Antibodies","PeriodicalId":55880,"journal":{"name":"中华地方病学杂志","volume":"60 1","pages":"278-281"},"PeriodicalIF":0.0000,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of Yersinia pestis detection result of Citellus undulates and parasitic fleas in Gurt, Usu in Xinjiang, 2013\",\"authors\":\"Wang Xinhui, A. Bulikemuabudurexiti, S. Shi, A. Bulimitimaituohuti, L. Bing, A. Zhatirehemu, Re Natuerdi, Xu Bingchen\",\"doi\":\"10.3760/CMA.J.ISSN.2095-4255.2015.04.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo observe immunological and bacteriological dynamic and distribution of Yersinia pestis in Citellus undulates in Gurt plague natural focus in Usu, Xinjiang in 2013. \\n \\n \\nMethods \\nIn the focus,Citellus undulates was captured with cram frame and blood from heart was collected and serum was isolated from June to August 2013. Indirect hemagglutination test (IHA) and enzyme-linked immunosorbent assay (ELISA) were taken to detect F1 antibody against Yersinia pestis of Citellus undulates, and the accuracy of two methods was conducted. Parasitic fleas were collected and classified. Yersinia pestis was isolated with four-step method (microscopic examination, cultivation for Yersinia pestis, bacteriovirus test and animal experiment) from liver, spleen of mice and parasitic fleas. \\n \\n \\nResults \\nTotally 436 Citellus undulates were captured and 2 801 parasitic fleas were collected. Positive rate of F1 antibody was 19.0% (83/436) and 4.4% (19/436) by ELISA and IHA, geometric mean titer (GMT) was 1∶26.92 and 1∶23.17, respectively. The positive rate and GMT of ELISA was higher than IHA (χ2 = 45.44,t = 1.96,P < 0.01 or < 0.05). The positive rate of F1 antibody was 20.2% (38/188), 25.9% (33/127) and 9.9% (12/121) from June, July to August, respectively, and the difference between months was statistically significant (χ2 = 10.67,P < 0.01). Totally 32 strains of Yersinia pestis were isolated. From June to August, the isolated strains were accounted for 81.3% (26/32), 12.5% (4/32) and 6.3% (2/32), respectively, the difference between months was statistically significant (χ2 = 20.60,P < 0.01). The ratio of strains isolated from 3 kinds of samples was 56.3% (18/32) of the fleas, 25.0% (8/32) of self-dead rodents and 18.8% (6/32) of the captured rodents, the difference between samples was statistically significant (χ2 = 18.46,P < 0.01). \\n \\n \\nConclusions \\nELISA is better than IHA for detecting sera F1 antibody, and testing accuracy can be improved by combination of the two methods. Strain detection rate is higher in June, and positive rate of self dead rodents is higher than that of captured rodents and fleas. More attention should be paid to strains isolation from the fleas in plague surveillance. Searching for self dead rodents is an effective way for strains isolation. \\n \\n \\nKey words: \\nCitellus undulates; Parasitic fleas; Yersinia pestis; Antibodies\",\"PeriodicalId\":55880,\"journal\":{\"name\":\"中华地方病学杂志\",\"volume\":\"60 1\",\"pages\":\"278-281\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-04-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华地方病学杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.2095-4255.2015.04.012\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华地方病学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.2095-4255.2015.04.012","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

摘要

目的观察2013年新疆乌苏Gurt鼠疫自然疫源地波状黄虫鼠疫耶尔森菌的免疫学和细菌学动态及分布情况。方法2013年6 - 8月，采用夹心法捕鼠，取心血，分离血清。采用间接血凝试验(IHA)和酶联免疫吸附试验(ELISA)检测波状黄霉鼠疫耶尔森菌F1抗体，并对两种方法的准确性进行了比较。收集寄生虫并进行分类。采用显微镜检查、鼠疫菌培养、细菌病毒试验和动物实验四步法从小鼠肝脏、脾脏和寄生蚤中分离出鼠疫菌。结果共捕获黄鼠436只，寄生蚤2 801只。ELISA和IHA检测F1抗体阳性率分别为19.0%(83/436)和4.4%(19/436)，几何平均滴度(GMT)分别为1∶26.92和1∶23.17。ELISA的阳性率和GMT均高于IHA (χ2 = 45.44,t = 1.96,P < 0.01或< 0.05)。6、7、8月血清F1抗体阳性率分别为20.2%(38/188)、25.9%(33/127)、9.9%(12/121)，月份间差异有统计学意义(χ2 = 10.67,P < 0.01)。共分离鼠疫耶尔森菌32株。6 ~ 8月分离株数分别为81.3%(26/32)、12.5%(4/32)和6.3%(2/32)，月份间差异有统计学意义(χ2 = 20.60,P < 0.01)。3种样本中分离出的蚤类、自死鼠和捕获鼠分别为56.3%(18/32)、25.0%(8/32)和18.8%(6/32)，样本间差异有统计学意义(χ2 = 18.46,P < 0.01)。结论ELISA法检测血清F1抗体优于IHA法，两种方法联用可提高检测准确性。6月鼠种检出率较高，自死鼠阳性率高于捕获鼠和蚤阳性率。在鼠疫监测中应更加重视从蚤体中分离菌株。寻找自死鼠是一种有效的菌株分离方法。关键词:黄芩;寄生蚤;鼠疫杆菌;抗体

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Analysis of Yersinia pestis detection result of Citellus undulates and parasitic fleas in Gurt, Usu in Xinjiang, 2013

Objective To observe immunological and bacteriological dynamic and distribution of Yersinia pestis in Citellus undulates in Gurt plague natural focus in Usu, Xinjiang in 2013. Methods In the focus,Citellus undulates was captured with cram frame and blood from heart was collected and serum was isolated from June to August 2013. Indirect hemagglutination test (IHA) and enzyme-linked immunosorbent assay (ELISA) were taken to detect F1 antibody against Yersinia pestis of Citellus undulates, and the accuracy of two methods was conducted. Parasitic fleas were collected and classified. Yersinia pestis was isolated with four-step method (microscopic examination, cultivation for Yersinia pestis, bacteriovirus test and animal experiment) from liver, spleen of mice and parasitic fleas. Results Totally 436 Citellus undulates were captured and 2 801 parasitic fleas were collected. Positive rate of F1 antibody was 19.0% (83/436) and 4.4% (19/436) by ELISA and IHA, geometric mean titer (GMT) was 1∶26.92 and 1∶23.17, respectively. The positive rate and GMT of ELISA was higher than IHA (χ2 = 45.44,t = 1.96,P < 0.01 or < 0.05). The positive rate of F1 antibody was 20.2% (38/188), 25.9% (33/127) and 9.9% (12/121) from June, July to August, respectively, and the difference between months was statistically significant (χ2 = 10.67,P < 0.01). Totally 32 strains of Yersinia pestis were isolated. From June to August, the isolated strains were accounted for 81.3% (26/32), 12.5% (4/32) and 6.3% (2/32), respectively, the difference between months was statistically significant (χ2 = 20.60,P < 0.01). The ratio of strains isolated from 3 kinds of samples was 56.3% (18/32) of the fleas, 25.0% (8/32) of self-dead rodents and 18.8% (6/32) of the captured rodents, the difference between samples was statistically significant (χ2 = 18.46,P < 0.01). Conclusions ELISA is better than IHA for detecting sera F1 antibody, and testing accuracy can be improved by combination of the two methods. Strain detection rate is higher in June, and positive rate of self dead rodents is higher than that of captured rodents and fleas. More attention should be paid to strains isolation from the fleas in plague surveillance. Searching for self dead rodents is an effective way for strains isolation. Key words: Citellus undulates; Parasitic fleas; Yersinia pestis; Antibodies

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.