光敏色素对萝卜幼苗基因表达的控制。远红光介导的多核糖体形成和可翻译mrna的出现

Pierre Fourcroy, Claude Lambert , Dominique Klein-Eude
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引用次数: 5

摘要

远红光照射萝卜幼苗,子叶和下胚轴的多核糖体含量增加。从黄化和远红光处理的萝卜幼苗子叶中分离出含有Poly(A)的RNA,并在兔网织细胞裂解液中翻译。翻译产物的比较表明,辐照苗提取物中6种丰富多肽的可翻译mRNA编码相对水平较高。我们还研究了mRNA群体在器官特异性、多核糖体定位和对帽类似物7-甲基鸟苷5 ' -磷酸(pm7G)的抑制作用方面的比较。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochrome control of gene expression in radish seedlings I. Far-red light mediated stimulation of polyribosome formation and appearance of translatable mRNAs

Illumination of radish seedlings with far-red light brings about an increase of the polyribosome content of cotyledons and hypocotyls. Poly(A)-containing RNA from cotyledons of both etiolated and far-red light-treated radish seedlings were isolated and translated in a rabbit reticulocyte lysate. The comparison of translation products showed that the relative levels of translatable mRNA coding for six abundant polypeptides were higher in the extracts of irradiated seedlings. The comparison of mRNA populations was also investigated with respect to organ specificity, polyribosomal localization and inhibition with the cap analoge, 7-methylguanosine 5′-phosphate (pm7G).

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