脱氧核糖核酸钠和口咽微生物群对1 ~ 6岁复发性呼吸道感染儿童的免疫调节治疗

Q4 Medicine

Pediatriya - Zhurnal im G.N. Speranskogo Pub Date : 2023-08-10 DOI:10.24110/0031-403x-2023-102-4-166-171

T. I. Kurdyukova, O. Krasnorutskaya, D.Iu. Bugrimov, A. Shevtsov, G. Golosnaya

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引用次数: 0

摘要

呼吸道感染是目前的主要疾病之一，其中近三分之二的感染过程发生在儿童时期。反复呼吸道感染的发病机制是基于免疫系统环节的失败，主要是由于鼻咽和口咽粘膜免疫稳态的破坏。本研究的目的是确定使用脱氧核糖核酸钠的免疫调节治疗对反复呼吸道感染儿童口咽部微生物群的影响。本研究于2021年5月至11月对90例1 ~ 6岁的复发性呼吸道感染患儿进行单中心随机安慰剂对照封闭实验研究。2021年，在位于俄罗斯沃罗涅日的私人医疗中心“现代儿科中心”的基础上。主要组46例，男23例，女23例，年龄4岁[3];[5] [y/o]，经鼻给予脱氧核糖核酸钠药物30天。对照组44例(男22例，女22例，年龄4岁;5] y/o)按照相同的处方方案给予安慰剂(无菌0.1%氯化钠液体)。采用酶免疫分析法测定分泌性免疫球蛋白的浓度;通过16S核糖体RNA基因的基因测序研究微生物群的组成。两组的所有参与者都接受了两次检查:在研究开始时和90天后。在使用该药物的背景下，发现变形菌门的相对数量减少，厚壁菌门和放线菌门的相对数量增加，sIgA浓度增加，这反过来表明粘膜免疫活性增加(p<0.001)。结论:因此，1 ~ 6岁反复呼吸道感染患儿使用脱氧核糖核酸钠可导致口咽部变形菌门的相对数量减少，厚壁菌门和放线菌门的相对数量增加，具有统计学意义;粘膜免疫活性增加，sIgA浓度增加就是证据。

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IMMUNOMODULATORY THERAPY USING SODIUM DEOXYRIBONUCLEATE AND THE OROPHARYNGEAL MICROBIOTA IN CHILDREN AGED 1 TO 6 YEARS OLD WITH RECURRENT RESPIRATORY INFECTIONS

Respiratory infections are one of the leading diseases currently and almost 2/3 of these infectious processes occur in childhood. The pathogenesis of recurrent respiratory infections is based on the failure of the links of the immune system, mainly caused by a violation of the immune homeostasis of the mucous membranes of the nasopharynx and oropharynx. The purpose of this research was to determine the effect of the immunomodulatory therapy using sodium deoxyribonucleate on the microbiota of the oropharynx in children with recurrent respiratory infections. A single-center randomized placebo-controlled closed experimental study was performed in 90 pediatric patients with recurrent respiratory infections aged 1 to 6 y/o in May 2021-Nov. 2021 on the basis of the private medical center “Center for Modern Pediatrics” located in Voronezh, Russia. The main group consisted of 46 (23 boys and 23 girls aged 4 [3; 5] y/o) who had been given the sodium deoxyribonucleate drug nasally for 30 days. The control group of 44 (22 boys and 22 girls aged 4 [3; 5] y/o) had been given a placebo (a sterile 0.1% sodium chloride fluid) according to the same prescription scheme. The concentration of secretory immunoglobulin was determined by the enzyme immunoassay; the composition of the microbiota was studied by the genetic sequencing of the 16S ribosomal RNA gene. All of the participants in both groups were examined twice: at the start of the study and 90 days after. A statistically significant decrease in the relative amount of Proteobacteria and an increase in the relative amount of Firmicutes and Actinobacteriota, an increase in the concentration of sIgA was found against the background of the use of the drug, which in tis turn indicates an increase in the activity of mucosal immunity (p<0.001). Conclusion: thus, the use of sodium deoxyribonucleate in children aged 1 to 6 years old with recurrent respiratory infections leads to a statistically significant decrease in the relative amount of Proteobacteria and an increase in the relative amount of Firmicutes and Actinobacteriota in the oropharynx; increased activity of mucosal immunity, as evidenced by an increase in the concentration of sIgA.

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来源期刊

Pediatriya - Zhurnal im G.N. Speranskogo Medicine-Pediatrics, Perinatology and Child Health

CiteScore

0.60

自引率

0.00%

发文量

150

期刊介绍： Journal “Pediatria” named after G.N. Speransky (the official short names of the Journal are “Journal «Pediatria»,” “Pediatria,” and “«Pediatria,» the Journal”) is the oldest Soviet-and-Russian (in the Russian Federation, the CIS and former Soviet Union) scientific and practical medical periodical assigned for pediatricians that is published continuously since May, 1922, and distributed worldwide. Our mission statement specifies that we aim to the ‘raising the level of skills and education of pediatricians, organizers of children’s health protection services, medicine scientists, lecturers and students of medical institutes for higher education, universities and colleges worldwide with an emphasis on Russian-speaking audience and specific, topical problems of children’s healthcare in Russia, the CIS, Baltic States and former Soviet Union Countries and their determination with the use of the World’s best practices in pediatrics.’ As part of this objective, the Editorial of the Journal «Pediatria» named after G.N. Speransky itself adopts a neutral position on issues treated within the Journal. The Journal serves to further academic discussions of topics, irrespective of their nature - whether religious, racial-, gender-based, environmental, ethical, political or other potentially or topically contentious subjects. The Journal is registered with the ISSN, - the international identifier for serials and other continuing resources, in the electronic and print world: ISSN 0031-403X (Print), and ISSN 1990-2182 (Online). The Journal was founded by the Academician, Dr. Georgiy Nestorovich SPERANSKY, in May, 1922. Now (since 1973) the Journal bears his honorary name.