评估实验性慢性子宫内膜炎模型的放射治疗效果和调节类固醇生成

Q3 Medicine
A. E. Chernova, O. V. Remneva, I. Bobrov, A. V. Lepilov, O. Mazko, O. Makarova, N. M. Semenikhina, O. V. Rozhkova, I. V. Grebtsov, D. Baranov, S. Antonova, V. V. Baranova
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Experimental group animals (n = 10) received a wide-spectrum antibiotic Ceftriaxone within 7 days followed by applying 10 procedures of radon therapy one a day and from day 41, the first procedure lasting 5 minutes, the second – 8 minutes, the third – 10 minutes, with all other procedures – for 15 minutes. Comparison group I animals (n = 10) received antibacterial therapy, radon therapy and 10 procedures of peloid therapy with natural salt-saturated medium sulfide silt therapeutic mud according to a radon therapy regimen. Сomparison group II (n = 10) received antibacterial and peloid therapy according to comparison group I regimen. Control group (n = 10) received no treatment. Inflammatory signs were assessed by endometrium histology study. The venous steroid hormones levels were analyzed by enzyme-linked immunosorbent assay.Results. 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引用次数: 0

摘要

目的:观察氡对慢性子宫内膜炎模型的治疗效果及激素的调节作用。材料和Мethods。实验研究了氡矿泉水的治疗效果。水中氡浓度为5.4 ~ 6.2 nCi/dm3。实验用Wistar系大鼠(n = 40)接种子宫内自粪悬浮液诱导实验性子宫内膜炎。实验组动物(n = 10)在7天内接受广谱抗生素头孢曲松治疗,随后进行10次氡治疗,每天一次,从第41天开始,第一次持续5分钟,第二次持续8分钟,第三次持续10分钟,所有其他程序持续15分钟。对照组(n = 10)按氡治疗方案进行抗菌治疗、氡治疗和天然盐饱和介质硫化物淤泥治疗泥10道类球治疗。Сomparison II组(n = 10)按对照组1方案进行抗菌及类球治疗。对照组(n = 10)不进行治疗。通过子宫内膜组织学检查评估炎症征象。采用酶联免疫吸附法分析静脉类固醇激素水平。实验组与对照组相比,发现子宫内膜弥漫性浸润密度较低,分别为125.4±8.1和180.7±9.0个免疫能细胞(30.7%;p = 0.002),而比较I组炎症浸润密度最小(与对照组相比减少了88.7%;P < 0.001),达到20.5±1.8个细胞。实验组及比较I、II组治疗后血清孕酮水平较对照组显著升高(p = 0.008)。实验组采用氡治疗时,孕酮水平较对照II组采用类黄酮治疗时升高更为明显。主要对照组经氡治疗后雌二醇水平明显下降(p = 0.008),而对照组I组和II组雌二醇水平与对照组相比无明显变化。氡对慢性子宫内膜炎模型有抗炎作用。氡治疗联合类球治疗有增强作用。使用氡疗法时,孕酮水平明显升高,雌二醇水平明显下降,建议进行临床试验,以确认其对生殖功能障碍包括伴有高雌激素血症的疾病的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessing radonotherapy effectiveness and modulated steroidogenesis in experimental chronic endometritis model
Aim: to evaluate therapeutic efficacy and modulated steroidogenesis after radon therapy in experimental chronic endometritis model.Materials and Мethods. A therapeutic effect related to radon mineral waters was studied experimentally. The water radon concentration was 5.4–6.2 nCi/dm3. The experimental endometritis was induced in laboratory Wistar line rats (n = 40) inoculated with intra-uterine self-fecal suspension. Experimental group animals (n = 10) received a wide-spectrum antibiotic Ceftriaxone within 7 days followed by applying 10 procedures of radon therapy one a day and from day 41, the first procedure lasting 5 minutes, the second – 8 minutes, the third – 10 minutes, with all other procedures – for 15 minutes. Comparison group I animals (n = 10) received antibacterial therapy, radon therapy and 10 procedures of peloid therapy with natural salt-saturated medium sulfide silt therapeutic mud according to a radon therapy regimen. Сomparison group II (n = 10) received antibacterial and peloid therapy according to comparison group I regimen. Control group (n = 10) received no treatment. Inflammatory signs were assessed by endometrium histology study. The venous steroid hormones levels were analyzed by enzyme-linked immunosorbent assay.Results. In experimental vs. control group, it was found that endometrial diffuse infiltrate was of lower density comprising 125.4 ± 8.1 vs. 180.7 ± 9.0 immunocompetent cells (by 30.7%; p = 0.002), whereas in comparison group I, density of inflammatory infiltration was minimal (reduced by 88.7 % compared to control group; p < 0.001) and reached 20.5 ± 1.8 cells. In the experimental group as well as comparison groups I and II vs. control group, a significant increased level of serum progesterone was observed after treatment (p = 0.008). When using a radon therapy in experimental group, it resulted in a more prominent rise in progesterone level compared to peloid therapy in comparison group II. Radon balneotherapy was found in main control group to elicit a significant declined estradiol level (p = 0.008), but not in comparison groups I and II featured with no significant change in estradiol level compared to control group.Conclusion. Radon therapy exerts an anti-inflammatory effect in experimental chronic endometritis model. Radon therapy combined with peloid therapy has a potentiated effect. The use of radon balneotherapy is accompanied by significantly increased progesterone and decreased estradiol level suggesting to conduct clinical trials for confirming its effectiveness in patients with reproductive dysfunction including diseases accompanied by hyperestrogenism.
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