辐照牛肝线粒体DNA的变化:一种检测方法

E. Marchioni , M. Tousch , V. Zumsteeg , F. Kuntz , C. Hasselmann
{"title":"辐照牛肝线粒体DNA的变化:一种检测方法","authors":"E. Marchioni ,&nbsp;M. Tousch ,&nbsp;V. Zumsteeg ,&nbsp;F. Kuntz ,&nbsp;C. Hasselmann","doi":"10.1016/1359-0197(92)90213-Y","DOIUrl":null,"url":null,"abstract":"<div><p>The study of the radio degradation of DNA is one of a number of phenomena being investigated to develop methods for identifying irradiated foods. The specific behaviour under radiation of the mitochondrial DNA from beef liver gives the possibility of detecting if the product has been irradiated or not. An identification method could finally be developed. The appraisal of the mitochondrial supercoiled DNA fraction constitutes an unambiguous detection test for beef liver irradiation. A total of 120 different samples were irradiated at 5 different doses. The dose limit of detection is lower than 2 kGy. No effects due to storage conditions were observed.</p></div>","PeriodicalId":14262,"journal":{"name":"International Journal of Radiation Applications and Instrumentation. Part C. Radiation Physics and Chemistry","volume":"40 6","pages":"Pages 485-488"},"PeriodicalIF":0.0000,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/1359-0197(92)90213-Y","citationCount":"3","resultStr":"{\"title\":\"Alterations of mitochondrial DNA: A method for the detection of irradiated beef liver\",\"authors\":\"E. Marchioni ,&nbsp;M. Tousch ,&nbsp;V. Zumsteeg ,&nbsp;F. Kuntz ,&nbsp;C. Hasselmann\",\"doi\":\"10.1016/1359-0197(92)90213-Y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The study of the radio degradation of DNA is one of a number of phenomena being investigated to develop methods for identifying irradiated foods. The specific behaviour under radiation of the mitochondrial DNA from beef liver gives the possibility of detecting if the product has been irradiated or not. An identification method could finally be developed. The appraisal of the mitochondrial supercoiled DNA fraction constitutes an unambiguous detection test for beef liver irradiation. A total of 120 different samples were irradiated at 5 different doses. The dose limit of detection is lower than 2 kGy. No effects due to storage conditions were observed.</p></div>\",\"PeriodicalId\":14262,\"journal\":{\"name\":\"International Journal of Radiation Applications and Instrumentation. Part C. Radiation Physics and Chemistry\",\"volume\":\"40 6\",\"pages\":\"Pages 485-488\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/1359-0197(92)90213-Y\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Radiation Applications and Instrumentation. Part C. Radiation Physics and Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/135901979290213Y\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Radiation Applications and Instrumentation. Part C. Radiation Physics and Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/135901979290213Y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

摘要

研究DNA的放射性降解是研究辐照食品鉴别方法的众多现象之一。牛肝线粒体DNA在辐射下的特定行为使检测产品是否受到辐射成为可能。最终可以开发出一种鉴定方法。线粒体超螺旋DNA片段的鉴定构成了牛肝辐照的明确检测试验。总共有120个不同的样品被以5种不同的剂量照射。检测的剂量限低于2kgy。没有观察到由于储存条件的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alterations of mitochondrial DNA: A method for the detection of irradiated beef liver

The study of the radio degradation of DNA is one of a number of phenomena being investigated to develop methods for identifying irradiated foods. The specific behaviour under radiation of the mitochondrial DNA from beef liver gives the possibility of detecting if the product has been irradiated or not. An identification method could finally be developed. The appraisal of the mitochondrial supercoiled DNA fraction constitutes an unambiguous detection test for beef liver irradiation. A total of 120 different samples were irradiated at 5 different doses. The dose limit of detection is lower than 2 kGy. No effects due to storage conditions were observed.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信