橙皮苷和薯蓣皂苷对坏死性小肠结肠炎的作用:实验研究

IF 0.4 4区医学 Q4 PEDIATRICS

Iranian Journal of Pediatrics Pub Date : 2023-02-10 DOI:10.5812/ijp-127271

U. Şenel, Halil İbrahim Tanrıverdi, S. Tanrıverdi, Hilal Irmak Sapmaz, Ali Akbaş, F. Gevrek, M. Uysal, U. Taş

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引用次数: 0

摘要

背景:坏死性小肠结肠炎(NEC)是新生儿中一种严重的肠道疾病。目的:探讨橙皮苷(Hsd)和地奥明对NEC的潜在保护作用。方法:将30只新生大鼠分为3组:对照组(n = 10)、NEC组(n = 10)和治疗组(n = 10)。治疗组大鼠给予黄酮类化合物100 mg/kg口服灌胃，每日2次。实验第5天，在麻醉下处死动物。剖腹手术后，取胃、盲肠、回肠组织标本。组织病理学上进行NEC评分。TUNEL(末端脱氧核苷酸转移酶介导的生物素-脱氧尿苷三磷酸镍端标记)染色评价细胞凋亡变化。此外，通过组织的生化分析确定了氧化剂和抗氧化剂的水平。结果:在检查的所有胃肠道区域，NEC组谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)水平均显著高于对照组。与对照组相似，黄酮类化合物处理组仅在盲肠中发现GSH-Px和MDA较低。NEC组3个区域的NEC损伤评分和凋亡指数均显著高于对照组和治疗组。治疗组胃和盲肠的凋亡指数与对照组相似，仅盲肠的NEC损伤评分与对照组相似。结论:Hsd和地奥司明治疗可显著降低nec诱导的损伤和凋亡细胞死亡的严重程度，特别是在盲肠。

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Effects of Hesperidin and Diosmin on Necrotizing Enterocolitis: An Experimental Study

Background: Necrotizing enterocolitis (NEC) is a serious intestinal illness in newborns. Objectives: We aimed to investigate the potential protective effects of hesperidin (Hsd) and diosmin on NEC. Methods: Thirty newborn rats were divided into 3 groups: The control group (n = 10), the NEC group (n = 10), and the treatment group (n = 10). The treatment group was given 100 mg/kg of the flavonoid by oral gavage twice daily. On day 5 of the study, animals were sacrificed under anesthesia. After laparotomy, the tissue samples were obtained from the stomach, cecum, and ileum. NEC scoring was performed histopathologically. Apoptotic changes were evaluated by TUNEL (terminal deoxynucleotidyl transferase–mediated biotin–deoxyuridine triphosphate nick-end labeling) staining. Furthermore, levels of oxidants and antioxidants were determined by biochemical analyzes of the tissues. Results: Glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) levels were significantly higher in the NEC group than in the control group in all gastrointestinal tract regions examined. Similar to the control group, GSH-Px and MDA were found to be low only in the cecum in the group treated with flavonoids. NEC damage score and apoptotic index in all 3 regions examined were significantly higher in the NEC group than in the control and treatment groups. The apoptotic index values in the treatment group were similar in the stomach and cecum, and the NEC damage score was similar to those in the control group only in the cecum. Conclusions: Hsd and diosmin treatment significantly reduces the severity of NEC-induced damage and apoptotic cell death, especially in the cecum.

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来源期刊

Iranian Journal of Pediatrics 医学-小儿科

CiteScore

0.90

自引率

20.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Iranian Journal of Pediatrics (Iran J Pediatr) is a peer-reviewed medical publication. The purpose of Iran J Pediatr is to increase knowledge, stimulate research in all fields of Pediatrics, and promote better management of pediatric patients. To achieve the goals, the journal publishes basic, biomedical, and clinical investigations on prevalent diseases relevant to pediatrics. The acceptance criteria for all papers are the quality and originality of the research and their significance to our readership. Except where otherwise stated, manuscripts are peer-reviewed by minimum three anonymous reviewers. The Editorial Board reserves the right to refuse any material for publication and advises that authors should retain copies of submitted manuscripts and correspondence as the material cannot be returned. Final acceptance or rejection rests with the Editors.