金合花根提取物对人白细胞和红细胞抗过氧化氢氧化损伤的有益作用

P. Mali
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引用次数: 7

摘要

背景:由于自由基负荷增加和/或抗氧化系统效率降低而导致的氧化损伤与许多人类疾病有关。马鞭草(马鞭草科)是一种重要的木本药用植物,在阿育吠陀、悉陀和乌纳尼医学体系中占有突出地位。传统上,它已被用于各种抗氧化相关疾病。目的:探讨三合戟根提取物对人白细胞和红细胞抗过氧化氢(h2o2)氧化损伤的作用。材料与方法:采用三氯甲烷:甲醇(1:1)的三氯甲烷:甲醇提取物(CMEPI)和三氯甲烷根水提物测定过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽(GSH)和脂质过氧化(LPO)水平。结果:本研究结果显示,与对照组相比,h2o2组小鼠血清CAT、SOD、GPx水平升高,GSH、LPO水平降低。与h2o2组相比,连翘根提取物处理组的CAT、SOD、GPx含量降低,GSH和LPO含量升高。发现CMEPI比水溶液更有效。结论:本研究表明,三合戟根提取物对人白细胞和红细胞具有抗h2o2氧化损伤的作用,可作为抗氧化剂用于民族医药。观察到的效果可归因于植物中的类黄酮和酚含量。此外,其对机体抗氧化系统的影响还需要体外和体内研究,以证明其临床安全性、可靠性和有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Beneficial effect of extracts of Premna integrifolia root on human leucocytes and erythrocytes against hydrogen peroxide induced oxidative damage
Background: Oxidative damage as a result of an increase in the free radical load and/or decrease in the efficiency of the antioxidant systems has been implicated in many human diseases. Premna integrifolia (Verbenaceae) is an important woody, medicinal plant and has a prominent place in Ayurvedha, Siddha and Unani system of medicines. Traditionally, it has been used for various antioxidant related disorders. Objective: The objective of the present study was to evaluate the beneficial effect of extracts of P. integrifolia root on human leucocytes and erythrocytes against hydrogen peroxide (H 2 O 2 ) induced oxidative damage. Materials and Methods: Chloroform:methanol (1:1) extract of P. integrifolia (CMEPI) and aqueous extract of P. integrifolia roots were used to accessed catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione (GSH) and lipid peroxidation (LPO) levels in H 2 O 2 induced oxidative damage. Results: Results of the present study revealed that, there was an increase in the CAT, SOD, GPx and reduction of the GSH and LPO levels in H 2 O 2 group compared with the control. P. integrifolia root extract treated groups showed the reduction of CAT, SOD, GPx and increased in the GSH and LPO levels as compared with H 2 O 2 group. CMEPI was found to be more effective than aqueous. Conclusion: The present study suggests that, extracts of P. integrifolia root possess beneficial effect on human leucocytes and erythrocytes against H 2 O 2 induced oxidative damage which has substantiated their use in ethnomedicine as an antioxidant. Observed effect can be attributed due to the flavonoid and phenol contents in the plant. Furthermore, in-vitro and in-vivo studies are needed to explore its effects on antioxidant system of the body for proving its clinical safety, reliability and efficacy.
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