{"title":"利用FT-IR、HPLC和GC-MS分析研究黑醋子乙醇提取物的抗氧化活性","authors":"J. Sujatha, S. Asokan","doi":"10.22192/IJARBS.2017.04.12.012","DOIUrl":null,"url":null,"abstract":"The aim of the present investigation is to screening of phytochemical constituents for its free radical scavenging activity of ethanol extract of Cassia alata leaves. The preliminary screening was carried out by standard chemical method. It evidenced that the presence of phytochemicals such as alkaloids, carbohydrate, phenols, tannins, falvanoids, steroids, protein and sugar in the ethanol extract. Various functional groups like alcohols, phenols, carboxylic acid and nitro groups were associated with the extract were characterized using Fourier transform infrared spectroscopy (FTIR). A few common compounds available in the extract were identified by Gas chromatography – Mass spectrum (GC-MS) analysis. The DPPH free radical scavenging activity and 50 % inhibitory concentration (IC50) was calculated and compared with the standard synthetic drug BHT. The IC50 value of leaf extract was found to be 24.56 μg/ml respectively. This study revealed that C. alata ethanol extract of leaves has alternative drugs for free radical it could be acted as a novel source of free radical scavengers.","PeriodicalId":13830,"journal":{"name":"International Journal of Advanced Research in Biological Sciences","volume":"40 1","pages":"112-119"},"PeriodicalIF":0.0000,"publicationDate":"2017-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Studies on the antioxidant activity of ethanol extract of Cassis alata using FT-IR, HPLC and GC-MS analysis\",\"authors\":\"J. Sujatha, S. Asokan\",\"doi\":\"10.22192/IJARBS.2017.04.12.012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of the present investigation is to screening of phytochemical constituents for its free radical scavenging activity of ethanol extract of Cassia alata leaves. The preliminary screening was carried out by standard chemical method. It evidenced that the presence of phytochemicals such as alkaloids, carbohydrate, phenols, tannins, falvanoids, steroids, protein and sugar in the ethanol extract. Various functional groups like alcohols, phenols, carboxylic acid and nitro groups were associated with the extract were characterized using Fourier transform infrared spectroscopy (FTIR). A few common compounds available in the extract were identified by Gas chromatography – Mass spectrum (GC-MS) analysis. The DPPH free radical scavenging activity and 50 % inhibitory concentration (IC50) was calculated and compared with the standard synthetic drug BHT. The IC50 value of leaf extract was found to be 24.56 μg/ml respectively. This study revealed that C. alata ethanol extract of leaves has alternative drugs for free radical it could be acted as a novel source of free radical scavengers.\",\"PeriodicalId\":13830,\"journal\":{\"name\":\"International Journal of Advanced Research in Biological Sciences\",\"volume\":\"40 1\",\"pages\":\"112-119\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Advanced Research in Biological Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22192/IJARBS.2017.04.12.012\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Advanced Research in Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22192/IJARBS.2017.04.12.012","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Studies on the antioxidant activity of ethanol extract of Cassis alata using FT-IR, HPLC and GC-MS analysis
The aim of the present investigation is to screening of phytochemical constituents for its free radical scavenging activity of ethanol extract of Cassia alata leaves. The preliminary screening was carried out by standard chemical method. It evidenced that the presence of phytochemicals such as alkaloids, carbohydrate, phenols, tannins, falvanoids, steroids, protein and sugar in the ethanol extract. Various functional groups like alcohols, phenols, carboxylic acid and nitro groups were associated with the extract were characterized using Fourier transform infrared spectroscopy (FTIR). A few common compounds available in the extract were identified by Gas chromatography – Mass spectrum (GC-MS) analysis. The DPPH free radical scavenging activity and 50 % inhibitory concentration (IC50) was calculated and compared with the standard synthetic drug BHT. The IC50 value of leaf extract was found to be 24.56 μg/ml respectively. This study revealed that C. alata ethanol extract of leaves has alternative drugs for free radical it could be acted as a novel source of free radical scavengers.
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