培养蘑菇金针菇、绿环菌和猴头菌的细胞毒/细胞抑制作用

Nina Vedenicheva, Nina Bisko, Larysa Kot, L. Garmanchuk, L. Ostapchenko
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引用次数: 0

摘要

从天然原料中获得的生物制剂,特别是从蘑菇中获得的生物制剂,在临床和制药实践中的应用具有迫切的重要性。目前,药用蘑菇的培养技术被广泛应用,其菌丝体中含有大量的细胞分裂素,细胞分裂素是多种植物细胞增殖和分化的刺激因子,在动物和人体内也具有免疫调节和抑瘤作用。因此,本研究的目的是确定金针菇、绿环菌和猴头菌的细胞毒/细胞抑制作用。细胞分裂素的纯化分阶段进行,采用离心、正丁醇分离、Dowex 50Wx8柱离子交换层析和silagel 60 F254薄层析。在Agilent Zorbax Eclipse XDB-C18色谱柱上,采用质谱(Agilent 1200 LC,美国)反相液相色谱法对细胞分裂素进行鉴定和定量。采用MTT法对人宫颈癌HeLa细胞进行细胞毒/细胞抑制剂筛选,并对活细胞与死细胞的比例进行常规计数。研究结果表明,每g干重中细胞分裂素含量最高的是培养蘑菇H. erinaceus。此外,H. erinaceus对HeLa系人宫颈癌细胞的毒性最高,而蘑菇F. velutipes和C. aegerita表现出细胞抑制(抗增殖)作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CYTOTOXIC / CYTOSTATIC EFFECT OF CULTIVATED MUSHROOMS FLAMMULINA VELUTIPES, CYCLOCYBE AEGERITA AND HERICIUM ERINACEUS
The use in clinical and pharmaceutical practice of biological preparations obtained from natural raw materials, in particular from mushrooms, is of urgent importance. Currently, the cultivation technologies of medicinal mushrooms are widely used, the mycelium of which contains a significant amount of cytokinins – stimulators of proliferation and differentiation of various types of plant cells, which also exhibit an immunomodulatory and oncostatic effects in the organism of animals and humans. Therefore, the aim of this work was to determine the cytotoxic/cytostatic effect of cultivated mushrooms Flammulina velutipes, Cyclocybe aegerita and Hericium erinaceus. Purification of cytokinins was carried out in stages, using methods of centrifugation, fractionation with n-butanol, ion exchange chromatography on Dowex 50Wx8 columns and thin-layer chromatography on Silicagel 60 F254. Identification and quantification of cytokinins was performed by by liquid chromatography in the reverse phase of MS (Agilent 1200 LC, USA) on an Agilent Zorbax Eclipse XDB-C18 column. Cytotoxic/cytostatic screening was performed on HeLa cells derived from human cervical cancer tumors using the MTT assay and routine counting of the ratio of live to dead cells. The results of the research showed that the highest content of cytokinins per 1 g of dry weight was characteristic of cultivated mushrooms of the species H. erinaceus. Also, H. erinaceus has the highest toxicity against human cervical cancer cells of the HeLa line, while the mushrooms F. velutipes and C. aegerita showed a cytostatic (antiproliferative) effect.
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