木霉对雷马唑亮紫5R和产蛋白红MX-5B的脱色作用

Vanessa Jane Zainip, Liyana Amalina Adnan, M. S. Elshikh
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引用次数: 13

摘要

工业废水包括染料废水的处理,已大量排放,难以降解,特别是合成染料。本研究从UTM森林的腐木中分离到10种不同类型的真菌,并将其标记为S1-S10。选用两种染料分别为Procion Red MX-5B (PRMX5B)和Remazol Brilliant Violet 5R (RBV5R)。筛选了这些真菌对两种染料的脱色能力,并进一步测试了它们在几种参数下在液体培养基中对染料的脱色能力;碳和氮源,初始pH值,温度和搅拌。S1在添加葡萄糖(45%)、硝酸铵(61%)、pH 3(69%)、温度37℃(49%)、搅拌100 rpm(69%)的条件下对PRMX5B进行了有效脱色,而S2在添加葡萄糖(60%)、硝酸铵(49%)、pH 3(70%)、温度37℃(46%)、搅拌100 rpm(74%)的条件下进行了有效脱色。S1在添加葡萄糖(80%)、硝酸铵(62%)、pH值3、温度37℃(75%)、搅拌100转/分(90%)的条件下对RBV5R进行了有效脱色,而S2在添加葡萄糖(52%)、硝酸铵(67%)、pH值3、温度37℃(75%)、搅拌100转/分(71%)的条件下进行了有效脱色。用紫外-可见分光光度计测定染料的脱色率。然后使用18sr RNA方法鉴定这些真菌。根据宏观和微观特征及多遗传树分析,真菌S1属于柯宁质木霉,真菌S2属于atroviride木霉。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Decolorization of Remazol Brilliant Violet 5R and Procion Red MX-5B by Trichoderma Species
Industrial wastewater including dye waste disposal, has been released in a massive amount and is difficult to degrade, especially synthetic dyes. In this study, 10 different types of fungi were isolated from a decayed wood in UTM forest and were labelled as S1-S10. Two dyes were chosen for this study, which were Procion Red MX-5B (PRMX5B) and Remazol Brilliant Violet 5R (RBV5R). These fungi were screened for their ability to decolor both dyes and further tested for their ability to decolor the dyes in liquid medium under several parameters; carbon and nitrogen sources, initial pH value, temperature, and agitation. S1 decolorized PRMX5B efficiently with the addition of glucose (45%), ammonium nitrate (61%), pH 3 (69%), temperature 37°C (49%), and agitation 100 rpm (69%), whereas S2 decolorized efficiently with the addition of glucose (60%), ammonium nitrate (49%), pH 3 (70%), temperature 37°C (46%), and agitation 100 rpm (74%). S1 demonstrated efficient decolorization of RBV5R with the addition of glucose (80%), ammonium nitrate (62%), pH 3, temperature 37°C (75%), and agitation 100 rpm (90%), whereas S2 demonstrated efficient decolorization with the addition of glucose (52%), ammonium nitrate (67%), pH 3, temperature 37°C (75%), and agitation 100 rpm (71%).The percentage of decolorization of dyes was measured by using a UV-Vis spectrophotometer. These fungi were then identified using the 18sr RNA method. Based on macroscopic and microscopic characteristics and a polygenetic tree, fungi S1 belong to Trichoderma koningiopsis and fungi S2 belong to Trichoderma atroviride. 
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