SYNTHESIS AND INVESTIGATION OF THE INFLUENCE OF NEW AMINO ACID DERIVATIVES WITH INDOLE AND BENZOXAZOLINONE MOIETIES ON THE RAPESEED (BRASSICA NAPUS L.) GROWTH IN VITRO

Rapeseed ( Brassica napus L . ) is one of the world’s most important sources of vegetable oil and protein meal used for food and for industrial purposes. Primary and secondary metabolites stored in rapeseed depend on many factors: growth regulators, temperature, water stress, nutrients (nitrogen, phosphorus, potassium, calcium, boron, sulfur) and their amount, soil acidity, evolution change, and plant diseases. Indole-3-acetic acid (IAA) is one of the most important natural auxins. 3-[2-Oxo-1,3-benzoxazol-3(2 H )-yl]propanoic acid is know as a synthetic auxin. The sodium salt of this compound, stilite-14, has been studied for 10 years while growing sugar beets in Lithuania. Natural and synthetic auxins are known to promote rapeseed root growth. New indole-3-acetic acid and 3-[2-oxo-1,3-benzoxazol-3(2 H )-yl]propanoic acid derivatives containing b,g-amino acid or 3-carboxy-5-oxopyrrolidine fragments from the corresponding hydrazides were synthesized. It was determined that 2-({2-[2-(1 H -indol-3-yl)acetyl]hydrazino}methyl)succinic acid (8.3 μM/l) increased the length of rapeseed roots in the MS medium by 1.6 times in comparison with rapeseed, which were grown in MS medium without growth regulators, whereas IAA decreased their length. Also, 2-({2-[2-(1 H -indol-3-yl)acetyl]hydrazino}methyl)succinic acid (8.3 mM/l) increased rapeseed biomass by 2 times in comparison with control sample. It was determined that 5-oxo-1-({3-[2-oxo-1,3-benzoxazol-3(2 H )-yl]propanoyl}amino)-3-pyrrolidinecarboxylic acid (6 mM/l) increased the length of rapeseed roots in the MS medium by 1.2 times in comparison with control sample, but the sodium salt of 3-(3-benzoxazolonyl)propanoic acid decreased their length at all concentrations. Also, 5-oxo-1-({3-[2-oxo-1,3-benzoxazol-3(2 H )-yl]propanoyl}amino)-3-pyrrolidinecarboxylic acid (1.5 mM/l) increased rapeseed biomass, but the sodium salt of 3-(3-benzoxazolonyl)propanoic acid (1.5 mM/l) decreased the biomass by 39.1 % in comparison with the control sample.  DOI: http://dx.doi.org/10.5755/j01.ct.64.2.6022