{"title":"不同禽源肠炎沙门氏菌系统发育树及基因序列比较","authors":"H. Labib","doi":"10.21608/EPSJ.2021.195781","DOIUrl":null,"url":null,"abstract":": Salmonella is bacterium causing high morbidity and mortality rates in the birds’. The present study was performed on a total of 100 samples from diseased and apparently healthy quail came from governorates (Giza, Cairo, Damietta and kafrelsheik). Collected samples included different organs (liver, heart, lung, and bone marrow). And a comparison between the genetic tree and gene sequences of Salmonella isolated from {chicken-ducks and rabbit}. The bacteriological examination revealed that out of 100 collected samples, 22 samples (22 %) were positive for Salmonella isolation. the serotyping of Salmonella species isolates showed a major variety of serotypes which included Salmonella Enteritidis (27.3%); Salmonella Typhimurium Senftenberg (22.8% for- each ); Salmonella Agona, (18.1%) and Salmonella Magherafelt (9%) . Results of the sensitivity testing of salmonella isolated from quail. Showed that (72.8%, 68.2%) of strains were resistant to Nalidixic acid and Streptomycin, but sensitive to Gentamicin, Trimethoprim-sulfamethoxazole, (68.2%) of strains. Also, all Salmonella isolates showed multidrug resistance. We selected three strains to be sequenced with accession number MT267777 to MT267779 and it resembled Salmonella enterica subsp. enterica serovar Enteritidis strain from China, Korea, and UK with 100% identitiy percent.","PeriodicalId":11662,"journal":{"name":"Egyptian Poultry Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"COMPARISON OF PHYLOGENIC TREE AND GENE SEQUENCE OF SALMONELLA ENTERITIDIS ISOLATED FROM DIFFERENT BIRDS\",\"authors\":\"H. Labib\",\"doi\":\"10.21608/EPSJ.2021.195781\",\"DOIUrl\":null,\"url\":null,\"abstract\":\": Salmonella is bacterium causing high morbidity and mortality rates in the birds’. The present study was performed on a total of 100 samples from diseased and apparently healthy quail came from governorates (Giza, Cairo, Damietta and kafrelsheik). Collected samples included different organs (liver, heart, lung, and bone marrow). And a comparison between the genetic tree and gene sequences of Salmonella isolated from {chicken-ducks and rabbit}. The bacteriological examination revealed that out of 100 collected samples, 22 samples (22 %) were positive for Salmonella isolation. the serotyping of Salmonella species isolates showed a major variety of serotypes which included Salmonella Enteritidis (27.3%); Salmonella Typhimurium Senftenberg (22.8% for- each ); Salmonella Agona, (18.1%) and Salmonella Magherafelt (9%) . Results of the sensitivity testing of salmonella isolated from quail. Showed that (72.8%, 68.2%) of strains were resistant to Nalidixic acid and Streptomycin, but sensitive to Gentamicin, Trimethoprim-sulfamethoxazole, (68.2%) of strains. Also, all Salmonella isolates showed multidrug resistance. We selected three strains to be sequenced with accession number MT267777 to MT267779 and it resembled Salmonella enterica subsp. enterica serovar Enteritidis strain from China, Korea, and UK with 100% identitiy percent.\",\"PeriodicalId\":11662,\"journal\":{\"name\":\"Egyptian Poultry Science Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Egyptian Poultry Science Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/EPSJ.2021.195781\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Poultry Science Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/EPSJ.2021.195781","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
COMPARISON OF PHYLOGENIC TREE AND GENE SEQUENCE OF SALMONELLA ENTERITIDIS ISOLATED FROM DIFFERENT BIRDS
: Salmonella is bacterium causing high morbidity and mortality rates in the birds’. The present study was performed on a total of 100 samples from diseased and apparently healthy quail came from governorates (Giza, Cairo, Damietta and kafrelsheik). Collected samples included different organs (liver, heart, lung, and bone marrow). And a comparison between the genetic tree and gene sequences of Salmonella isolated from {chicken-ducks and rabbit}. The bacteriological examination revealed that out of 100 collected samples, 22 samples (22 %) were positive for Salmonella isolation. the serotyping of Salmonella species isolates showed a major variety of serotypes which included Salmonella Enteritidis (27.3%); Salmonella Typhimurium Senftenberg (22.8% for- each ); Salmonella Agona, (18.1%) and Salmonella Magherafelt (9%) . Results of the sensitivity testing of salmonella isolated from quail. Showed that (72.8%, 68.2%) of strains were resistant to Nalidixic acid and Streptomycin, but sensitive to Gentamicin, Trimethoprim-sulfamethoxazole, (68.2%) of strains. Also, all Salmonella isolates showed multidrug resistance. We selected three strains to be sequenced with accession number MT267777 to MT267779 and it resembled Salmonella enterica subsp. enterica serovar Enteritidis strain from China, Korea, and UK with 100% identitiy percent.