Modified Extraction Method for Determination of Fatty Acids from Tuna’s Eye in Iranian Coasts of Persian Gulf and Oman Sea

A B S T R A C T Tuna fish belongs to Scombridae family which includes Sardinae, Macerels, Tuna and many other species. These fish are harvested in a 30 thousands kilometers area in Persian Gulf and are processed in conserve factories. About 20± 3 wet weight percent of Tuna fish are by product. These by product were studied to extract the high-value productions; marine oils rich of unsaturated multiple-band fatty acids, in which 100kg was obtained from Chabahar and transferred to Urmia for analysis. Extracting oil was made using the Dyer and Bligh standard method. The results showed that 80± 5 % of wet weight of eye wastes of Tunas fish was oil; profile of the extracted oils were made using gas chromatography. The results showed that eye wastes of Tuna fish oil had 21.47% saturated fatty acids, 25.70% single band unsaturated fatty acids, and the total value of the unsaturated multiple band was 40.99%. The fatty acid contents of arachidonic acid, linolenic acid, linoleic acid, eicosapentaenoic acid and docosahexaenoic acid were 1.78, 4.10, 6.20, 17.40 and 9.86% of the total fatty acids, respectively. Also, the results revealed that eye wastes of Tuna fish is considered for the