载烟熏有机提取物的PLGA纳米颗粒对卵巢癌细胞株A2780的抗癌作用

IF 2.9 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY

Materials Technology Pub Date : 2022-10-10 DOI:10.1080/10667857.2022.2133072

Roya Mahdizadeh, M. Firoozrai, M. Homayouni Tabrizi, S. J. Hoseini

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引用次数: 0

摘要

许多传统药物与新技术的结合可以产生有效的治疗效果。因此，本研究的目的是评估负载烟熏有机提取物(P-ASOE)的PLGA纳米颗粒(NPs)对卵巢癌细胞系A2780的作用。因此，采用W1/O/W2法合成纳米颗粒，并采用FESEM、DLS和FTIR技术进行表征。采用MTT法评价P-ASOE对A2780和健康(HFF)细胞的细胞毒性。采用吖啶橙/碘化丙啶(AO/PI)染色、流式细胞术及P53、TNFα、CAS-3和CAS-9基因表达谱研究P-ASOE对A2780细胞凋亡的影响。通过绒毛尿囊膜(CAM)测定及VEGF、VEGFR基因表达测定研究P-ASOE的抗血管生成作用。研究了P-ASOE对小鼠健康组织和癌变组织的组织学影响。结果表明，合成的NPs对A2780细胞具有剂量和时间依赖性的细胞毒作用，而对HFF细胞没有作用。结果表明，P-ASOE具有促凋亡作用，可显著减少鸡胚的血管数量和长度，显著减少鸡胚的长度和重量。在NPs的影响下，VEGF和VEGFR基因的表达水平也显著降低。根据体内检查，P-ASOE治疗减少了肿瘤大小，但对健康组织没有明显的组织学影响。这些结果都支持P-ASOE对A2780癌细胞的抗癌潜力。

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Anti-cancer effects of PLGA nanoparticles loaded with anbarnesa smoke organic extract on A2780 ovarian cancer cell line

ABSTRACT There are many traditional agents whose combination with new technologies can offer effective therapeutic impacts. Accordingly, the purpose of this study was to evaluate the effects of PLGA nanoparticles (NPs) loaded with anbarnesa smoke organic extract (P-ASOE) against A2780 ovarian cancer cell line. Thus, nanoparticles were synthesised using W1/O/W2 method and characterised by FESEM, DLS and FTIR techniques. Cytotoxicity of P-ASOE on A2780 and healthy (HFF) cells was assessed using MTT assay. Acridine Orange/Propidium Iodide (AO/PI) staining, flow cytometry and expression profiles of P53, TNFα, CAS-3 and CAS-9 genes were recruited to evaluate the effect of P-ASOE on apoptosis of A2780 cells. The antiangiogenic effect of P-ASOE was studied through chorioallantoic membrane (CAM) assay as well as evaluation of VEGF and VEGFR gene expression. The histological effects of P-ASOE on healthy and cancerous tissues of mouse models were investigated. The results showed that the synthesised NPs had dose- and time-dependent cytotoxic effects on A2780 cells but not on HFF cells. In addition, all assays indicated pro-apoptotic effects of P-ASOE, which significantly reduced number and length of blood vessels as well as length and weight of chick embryos. The expression levels of VEGF and VEGFR genes were also significantly decreased under the influence of NPs. According to in vivo examinations, P-ASOE treatment reduced tumour size while having no significant histological effect on healthy tissues. All findings were in favour of the anti-cancer potential of P-ASOE against A2780 cancer cell line.

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来源期刊

Materials Technology 工程技术-材料科学：综合

CiteScore

6.00

自引率

9.70%

发文量

105

审稿时长

8.7 months

期刊介绍： Materials Technology: Advanced Performance Materials provides an international medium for the communication of progress in the field of functional materials (advanced materials in which composition, structure and surface are functionalised to confer specific, applications-oriented properties). The focus is on materials for biomedical, electronic, photonic and energy applications. Contributions should address the physical, chemical, or engineering sciences that underpin the design and application of these materials. The scientific and engineering aspects may include processing and structural characterisation from the micro- to nanoscale to achieve specific functionality.