{"title":"大鼠胚胎细胞微囊泡和外泌体的分离与鉴定","authors":"V. Derevyannaya, S. Tseluyko","doi":"10.32743/2658-6460.2019.3.3.90","DOIUrl":null,"url":null,"abstract":"Exosomes - microvesicles with a size of 30-180 nm, which provide to intercellular communication between all or-gans and tissues. In this study we isolated exosomes and microvesicles from embrional rat`s cells, results were demon-strated by photographs of scanning electron microscopy to sample. In future we planning detect of exosomes by west-ern-blot with anti-CD63 antibody. Ключевые слова: экзосомы микровезикулы, экзосом, эмбриональные клетки.","PeriodicalId":8910,"journal":{"name":"Biological Journal of Microorganism","volume":"29 3 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"ISOLATION AND IDENTIFICATION MICROVESICLES AND EXOSOMES FROM EMBRRIONAL RAT`S CELL\",\"authors\":\"V. Derevyannaya, S. Tseluyko\",\"doi\":\"10.32743/2658-6460.2019.3.3.90\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Exosomes - microvesicles with a size of 30-180 nm, which provide to intercellular communication between all or-gans and tissues. In this study we isolated exosomes and microvesicles from embrional rat`s cells, results were demon-strated by photographs of scanning electron microscopy to sample. In future we planning detect of exosomes by west-ern-blot with anti-CD63 antibody. Ключевые слова: экзосомы микровезикулы, экзосом, эмбриональные клетки.\",\"PeriodicalId\":8910,\"journal\":{\"name\":\"Biological Journal of Microorganism\",\"volume\":\"29 3 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-03-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biological Journal of Microorganism\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32743/2658-6460.2019.3.3.90\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Journal of Microorganism","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32743/2658-6460.2019.3.3.90","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
摘要
外泌体-大小为30- 180nm的微囊泡，提供所有器官和组织之间的细胞间通讯。在本研究中，我们从胚胎大鼠细胞中分离出外泌体和微囊泡，并通过扫描电镜对样品进行了照片验证。未来我们计划用抗cd63抗体的western - western blot检测外泌体。Ключевыеслова:экзосомымикровезикулы,экзосом,эмбриональныеклетки。
ISOLATION AND IDENTIFICATION MICROVESICLES AND EXOSOMES FROM EMBRRIONAL RAT`S CELL
Exosomes - microvesicles with a size of 30-180 nm, which provide to intercellular communication between all or-gans and tissues. In this study we isolated exosomes and microvesicles from embrional rat`s cells, results were demon-strated by photographs of scanning electron microscopy to sample. In future we planning detect of exosomes by west-ern-blot with anti-CD63 antibody. Ключевые слова: экзосомы микровезикулы, экзосом, эмбриональные клетки.
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