叶龄、叶段和表面处理对高粱和枸杞炭疽病菌致病性的影响

GM crops Pub Date : 2021-10-26 DOI:10.3390/crops1030011
E. Ahn, Farrell Fan, C. Magill
{"title":"叶龄、叶段和表面处理对高粱和枸杞炭疽病菌致病性的影响","authors":"E. Ahn, Farrell Fan, C. Magill","doi":"10.3390/crops1030011","DOIUrl":null,"url":null,"abstract":"Colletotrichum sublineola is a casual pathogen of sorghum anthracnose. Sorghum pathologists often need to conduct evaluations for anthracnose resistance in large scale which are expensive and labor intensive. As a solution, an excised-leaf assay has been used, but whether or not leaf age, position or region affects pathogenicity scores has not previously been evaluated. Essentially, in an excised-leaf assay, is response to C. sublineola over all or part of a leaf blade identical? To get an answer, three sorghum and one Johnson grass cultivars were tested. The top five leaves were inoculated at the apex, mid-leaf and base of each leaf blade. Results show nearly no effect of leaf age to pathogenicity level within the top five leaves. Furthermore, in order to evaluate any protective role of leaf wax to C. sublineola, the wax was disrupted by simply wiping the leaf surface by a thumb, or as an alternative method, leaf surface tension was reduced by submerging leaves into 2% TWEEN 20 before inoculation. Compared to control, wiped leaves increased pathogenicity scores on the leaf blade and midrib in two of three sorghum cultivars, but 2% TWEEN 20-treated leaves had only minimal changes in pathogenicity level compared to controls.","PeriodicalId":89376,"journal":{"name":"GM crops","volume":"101 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Leaf Age, Leaf Segments and Surface Treatments on Pathogenicity Levels of Colletotrichum sublineola in Sorghum and Johnson Grass\",\"authors\":\"E. Ahn, Farrell Fan, C. Magill\",\"doi\":\"10.3390/crops1030011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Colletotrichum sublineola is a casual pathogen of sorghum anthracnose. Sorghum pathologists often need to conduct evaluations for anthracnose resistance in large scale which are expensive and labor intensive. As a solution, an excised-leaf assay has been used, but whether or not leaf age, position or region affects pathogenicity scores has not previously been evaluated. Essentially, in an excised-leaf assay, is response to C. sublineola over all or part of a leaf blade identical? To get an answer, three sorghum and one Johnson grass cultivars were tested. The top five leaves were inoculated at the apex, mid-leaf and base of each leaf blade. Results show nearly no effect of leaf age to pathogenicity level within the top five leaves. Furthermore, in order to evaluate any protective role of leaf wax to C. sublineola, the wax was disrupted by simply wiping the leaf surface by a thumb, or as an alternative method, leaf surface tension was reduced by submerging leaves into 2% TWEEN 20 before inoculation. Compared to control, wiped leaves increased pathogenicity scores on the leaf blade and midrib in two of three sorghum cultivars, but 2% TWEEN 20-treated leaves had only minimal changes in pathogenicity level compared to controls.\",\"PeriodicalId\":89376,\"journal\":{\"name\":\"GM crops\",\"volume\":\"101 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-10-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"GM crops\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/crops1030011\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"GM crops","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/crops1030011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

炭疽杆菌是高粱炭疽病的一种偶然病原菌。高粱病理学家经常需要进行大规模的炭疽病抗性鉴定,成本高,劳动强度大。作为一种解决方案,已经使用了一种切除的叶片测定法,但是叶片的年龄、位置或区域是否影响致病性评分以前没有被评估过。从本质上讲,在一个切除的叶片试验中,是整个叶片还是部分叶片的反应是相同的?为了得到答案,我们对三种高粱和一种庄稼草进行了试验。分别在每片叶片的先端、叶中和基部接种前5片叶片。结果表明,叶龄对前5叶的致病性水平几乎没有影响。此外,为了评估叶蜡对山核桃的保护作用,可以通过简单地用拇指擦拭叶片表面来破坏叶蜡,或者在接种前将叶片浸泡在2% TWEEN 20中来降低叶片表面张力。与对照相比,3个高粱品种中有2个品种的叶片经TWEEN 20处理后,叶片和中脉的致病性评分均有所提高,但与对照相比,2% TWEEN 20处理后叶片的致病性水平变化很小。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Leaf Age, Leaf Segments and Surface Treatments on Pathogenicity Levels of Colletotrichum sublineola in Sorghum and Johnson Grass
Colletotrichum sublineola is a casual pathogen of sorghum anthracnose. Sorghum pathologists often need to conduct evaluations for anthracnose resistance in large scale which are expensive and labor intensive. As a solution, an excised-leaf assay has been used, but whether or not leaf age, position or region affects pathogenicity scores has not previously been evaluated. Essentially, in an excised-leaf assay, is response to C. sublineola over all or part of a leaf blade identical? To get an answer, three sorghum and one Johnson grass cultivars were tested. The top five leaves were inoculated at the apex, mid-leaf and base of each leaf blade. Results show nearly no effect of leaf age to pathogenicity level within the top five leaves. Furthermore, in order to evaluate any protective role of leaf wax to C. sublineola, the wax was disrupted by simply wiping the leaf surface by a thumb, or as an alternative method, leaf surface tension was reduced by submerging leaves into 2% TWEEN 20 before inoculation. Compared to control, wiped leaves increased pathogenicity scores on the leaf blade and midrib in two of three sorghum cultivars, but 2% TWEEN 20-treated leaves had only minimal changes in pathogenicity level compared to controls.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信