基于itraq的定量蛋白质组学分析揭示了毛鳞螺原体侵袭3T6细胞的机制

IF 0.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Current Proteomics Pub Date : 2022-01-20 DOI:10.2174/1570164619666220113154423

Peng Liu, Youyuan Ye, Shasha Xiang, Yuxin Li, Chengbin Zhu, Zixu Chen, Jie Hu, Ye Gen, Li Lou, Xuqi Duan, Juan Zhang, W. Gu

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引用次数: 0

摘要

毛鳞螺原体是淡水甲壳类动物的一种新型病原体，与米氏螺原体有密切的亲缘关系。它们没有细胞壁，呈螺旋状。它们具有感染哺乳动物的能力，机制尚不清楚。在这项研究中，我们的目的是研究毛鳞棘球蚴感染3T6细胞的蛋白表达谱。应用itraq系统研究了鼠毛弧菌感染3T6细胞的蛋白质组学。我们鉴定并定量了4915个蛋白，发现差异蛋白67个，其中上调蛋白30个，下调蛋白37个。GO项分析表明，粘附蛋白、干扰素和细胞骨架调节的失调与细胞凋亡有关。粘附蛋白Vcam1和干扰素诱导蛋白GBP2、Ifit1、TAPBP、CD63、Arhgef2上调。关键的细胞骨架调节蛋白ARHGEF17下调。KEGG通路分析显示NF-kappa B信号通路、MAPK信号通路、jak - stat信号通路和nod样受体信号通路与体内细胞凋亡密切相关。分析侵染过程中涉及的信号通路可能为理解毛鳞棘球蚴的侵染机制提供新的思路。

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iTRAQ-Based Quantitative Proteomics Analysis Reveals the Invasion Mechanism of Spiroplasma eriocheiris in 3T6 Cells

Spiroplasma eriocheiris is a novel pathogen of freshwater crustaceans and is closely related to S. mirum. They have no cell wall and a helical morphology. They have the ability to infect mammals with an unclear mechanism. In this study, our aim was to investigate the profile of protein expression in 3T6 cells infected with S. eriocheiris. The proteome of 3T6 cells infected by S. eriocheiris was systematically investigated by iTRAQ. We identified and quantified 4915 proteins, 67 differentially proteins were found, including 30 up-regulated proteins and 37 down-regulated proteins. GO term analysis shows that dysregulation of adhesion protein , interferon and cytoskeletal regulation are associated with apoptosis. Adhesion protein Vcam1 and Interferon-induced protein GBP2, Ifit1, TAPBP, CD63 ,Arhgef2 were up-regulated. A key cytoskeletal regulatory protein, ARHGEF17 was down-regulated. KEGG pathway analysis showed the NF-kappa B signaling pathway, the MAPK signaling pathway , the Jak-STAT signaling pathway and NOD-like receptor signaling are closely related to apoptosis in vivo. Analysis of the signaling pathways involved in invasion may provide new insights for understanding the infection mechanisms of S. eriocheiris.

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来源期刊

Current Proteomics BIOCHEMICAL RESEARCH METHODS-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed in-depth/mini review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry. Current Proteomics publishes in-depth/mini review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, software, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to: Protein separation and characterization techniques 2-D gel electrophoresis and image analysis Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching Determination of co-translational and post- translational modification of proteins Protein/peptide microarrays Biomolecular interaction analysis Analysis of protein complexes Yeast two-hybrid projects Protein-protein interaction (protein interactome) pathways and cell signaling networks Systems biology Proteome informatics (bioinformatics) Knowledge integration and management tools High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography) High-throughput computational methods for protein 3-D structure as well as function determination Robotics, nanotechnology, and microfluidics.