利用扩增片段长度多态性揭示欧洲三种蛇舌草的遗传多样性

Magdalena Szeliga, Joanna Ciura, M. Tyrka
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引用次数: 2

摘要

摘要欧洲收集的Veratrum物种的化学和遗传特征对基因库保存和鉴定具有所需代谢特性的种群具有重要意义。Veratrum album, V. lobelianum和V. nigrum原产于欧洲,在波兰被列为稀有或濒危物种。利用扩增片段长度多态性(AFLP)标记对欧洲角曲属植物的遗传变异进行了分析。测定了以菊苣为代表的甾体生物碱在种子中的积累量。由8个引物组合得到的380个标记的分布,可用于研究Veratrum属植物种间和种内的亲缘关系。12个Veratrum种居群间的遗传变异支持了V. lobelianum作为V. album亚种的分类。然而,结果需要在扩展材料上进一步验证。种群间遗传多样性(22.3%)高于种群间遗传多样性(14.5%)。菊苣属植物的成分可作为一种潜在的化学分类标记。菊苣中菊苣的含量最高。在小红花种子中只检测到微量的菊苣素,未检测到菊苣素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic diversity of three European Veratrum species revealed by Amplified Fragment Length Polymorphism
Abstract Chemical and genetic characterization of Veratrum species deposited in European collections is important for genepool preservation and identification of populations with desired metabolic properties. Veratrum album, V. lobelianum and V. nigrum are native to Europe, and in Poland are ranked as rare or threatened. Genetic variation of European Veratrum species was characterized by Amplified Fragment Length Polymorphism (AFLP) markers. The accumulation of jervine as a representative of steroidal alkaloids was measured in seeds. Distribution of 380 markers generated from eight primer combinations was useful for studying genetic relationships among and within species in the Veratrum genus and the most divergent populations were identified. Genetic variation between 12 populations of Veratrum species supports the classification of V. lobelianum as a subspecies of V. album. However, the results need further validation on extended material. A higher genetic diversity (22.3%) was observed between populations of V. nigrum as compared to V. album (14.5%). Contents of jervine allowed for discrimination of the studied Veratrum species and can be used as a potential chemotaxonomic marker. The highest jervine levels were found in V. album. V. nigrum seeds had only trace amounts and no jervine was detected in seeds of V. lobelianum.
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