龙凤酒内生真菌产酚氧化酶的研究

S. B. Onofre, P. Steilmann, Francisc O Beltrão
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引用次数: 4

摘要

酚类化合物属于自然和人为生物降解产生的废物,存在于土壤和水中,但尽管在自然界中广泛分布,但它们是纺织、石油炼制、纸浆和造纸、制药、涂层金属、木材防腐剂、染料、塑料和树脂、煤炭转化等各种工业丢弃的主要污染物有毒废物的一部分,并且是许多杀菌剂的组成部分。为了弥补这些化合物的影响,研究了从龙骨龙骨杆菌属(Asteraceae)中分离的7种内生真菌产生能够降解酚类化合物的酚氧化酶的能力。将真菌接种于含有不同浓度没食子酸的培养基中,28℃孵育,每48 h监测一次。通过观察巴伐达姆反应特征的琥珀色晕来评估enzy me的产量。只有镰刀菌D3-FB和尾孢菌D7-FB在所有浓度下均出现降解晕。虽然在相同的培养基中发育,但其他物种没有表现出Bavendamm反应的迹象。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phenoloxidases Produced by Endophytic Fungi Isolated from Baccharis Dracunculifolia D. C. (Asteraceae)
Phenolic co mpounds fall within the waste resulting fro m the biodegradation of natural and anthropogenic, are found in soil and water, but despite being widely distributed in nature are part of the main pollutants toxic waste discarded by a wide variety of industries such as textiles, petroleum refining, pulp and paper, pharmaceuticals, coating metals, wood preservatives, dyes, plastics and resins, coal conversion, and are components of many biocides. In order to remedy the impacts of these compounds, seven endophytic fungal species isolated from Baccharis dracunculifolia D. C. (Asteraceae) were studied to determine their ability to produce phenoloxidases capable of degrading phenolic compounds. The fungi were inoculated in media containing different concentrations of gallic acid, incubated at 28℃ and mon itored every 48 h. En zy me production was assessed through the observation of an amber-co lored halo, which is characteristic of the Bavendamm's reaction. On ly Fusarium sp. strain D3-FB and Cercospora sp. strain D7-FB showed degradation halos at all concentrations. Although developed in the same med ia, the other species showed no signs of the Bavendamm's reaction.
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