酸枣和深海赤藓树皮粗提物对新隐球菌和白色念珠菌的抑菌活性

T. Manyarara, J. Chifamba, Felistas T. Tarugarira
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引用次数: 4

摘要

目的:从深海青霉属植物和毛霉属植物中提取、鉴定具有抗新生胞球菌和白色念珠菌活性的活性化合物。研究设计:用琼脂盘扩散法筛选植物粗提取物,确定其最低抑菌浓度。学习地点和时间:2014年2月和2015年5月,哈拉雷理工学院制药技术实验室。方法学:本研究采用紫茎草(Ziziphus mucronata)和深海赤藓(Erythrina abyssinica)粗树皮提取物;生物工程学报,10(3):1-11,2016;文章no.BJPR。采用圆盘扩散法,以氟康唑为阳性对照,对来自津巴布韦的23843 2株病原菌进行了对新型隐球菌、白色念珠菌的抑菌活性检测。对粗提物进行植物化学筛选,鉴定植物成分。结果:对白色念珠菌的最低有效抑菌浓度分别为20% w/v和10%w/v,在5% w/v浓度下呈中等生长。在25% w/v和12.5% w/v时,深海赤藓的最低抑菌浓度有效,在6.25% w/v时,真菌生长适中。两种粗提物在相同浓度范围内对新生梭菌的抑菌活性相似。两种粗树皮提取物均检测出单宁、皂苷、黄酮类化合物和生物碱的阳性,这些物质有助于植物物种的抗真菌活性。结论:白色念珠菌和新生隐球菌是引起HIV / AIDS患者机会性感染最常见的真菌种类。这些植物产生的效果已经证明,它们可以用来开发药物,减轻与这些感染有关的症状。植物粗提物对白色念珠菌和新型隐球菌有一定的抑制作用。两种粗树皮提取物均检测出单宁、皂苷、黄酮类化合物和生物碱的阳性,这些物质有助于植物物种的抗真菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antifungal Activity of Ziziphus mucronata and Erythrina abyssinica Bark Crude Extracts on Cryptococcus neofomans and Candida albicans Species
Aims: The study aims at extraction, characterization and identification of bioactive compounds with antifungal properties from the plant species E. abyssinica and Z. mucronata against Cyptococcal neoformans and Candida albicans. Study Design: Phytochemical screening of crude plant extracts and determination of their minimum inhibitory concentration using Agar disc diffusion method. Place and Duration of Study: Pharmaceutical Technology Lab, Harare Institute of Technology, February 2014 and May 2015. Methodology: In this study crude bark extracts of Ziziphus mucronata and Erythrina abyssinica Original Research Article Manyarara et al.; BJPR, 10(3): 1-11, 2016; Article no.BJPR.23843 2 from Zimbabwe were test for antifungal activity using the disc diffusion method against Cryptococcus neoformans, Candida albicans with fluconazole as the positive control. Phytochemical screening tests on the crude extracts were carried out to identify phytoconstituents. Results: Effective minimum inhibitory concentrations against C. albicans were found to be 20% w/v and 10%w/v for Ziziphus mucronata and showed moderate growth at a 5% w/v concentration. Erythrina abyssinica had effective minimum inhibitory concentrations at 25% w/v and 12.5% w/v with moderate fungal growth observed at 6.25% w/v. The same concentration ranges for both crude extracts showed similar antifungal activity for C. neoformans. Both crude bark extracts tested positive for tannins, saponins, flavonoids and alkaloids which contribute to the antifungal activity of the plant species. Conclusion: Candida albicans and Cryptococcus neoformans are the most common fungal species that cause opportunistic infections to occur in HIV and AIDS. The effects produced by these plants have proven that they can be used to develop pharmaceutical agents alleviate the symptoms associated with these infections. The crude plant extracts were found to be active against Candida albicans and Cryptococcus neoformans. Both crude bark extracts tested positive for tannins, saponins, flavonoids and alkaloids which contribute to the antifungal activity of the plant species.
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