膜脂与溶血因子溶血的关系(双翅目:蝇科)

H.J. Kirch , G. Spates , W.J. Kloft , J.R. DeLoach
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引用次数: 6

摘要

雌性稳定蝇在进食6种不同哺乳动物的溶血红细胞后12小时制备的后中肠匀浆比在22小时制备的匀浆更容易。红细胞膜鞘磷脂含量的百分比与12小时匀浆溶解所需的时间之间存在显著的相关性。低鞘磷脂含量的红细胞比高鞘磷脂含量的红细胞对溶解更敏感。22 h匀浆溶血不存在这种相关性。匀浆12 h和22 h时红细胞的平均体积和渗透脆性与溶血无关。磷脂酶C和鞘磷脂酶活性的测定表明,在12-14 h制备的匀浆中,磷脂酶C的水解率几乎是鞘磷脂酶活性的两倍。而在22-24 h的匀浆中,水解率与12-14 h的匀浆相比没有差异,且明显降低。红细胞溶血所需的时间与磷脂酶C和鞘磷脂酶活性谱有关,表明这些酶活性参与了红细胞的体外溶血。牛和人的红细胞暴露在中肠匀浆中时,它们的双凹形轮廓变成了针状球形。这种形状的变化被解释为一种清洁剂诱导的红细胞膜的修饰,使红细胞更容易溶血。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The relationship of membrane lipids to species specific hemolysis by hemolytic factors from Stomoxys calcitrans (L.) (Diptera: Muscidae)

Posterior-midgut homogenate from female stable flies prepared at 12 h after feeding hemolyzed erythrocytes from 6 different mammalian species more readily than homogenate prepared at 22 h. A significant correlation was obtained between the per cent sphingomyelin content of the erythrocyte membrane and the time required for lysis by the 12 h homogenate. Erythrocytes with low sphingomyelin content were more sensitive to lysis than cells with high sphingomyelin. No such correlation exists for hemolysis by 22 h homogenate. Mean corpuscular volume and osmotic fragilities of erythrocytes were not related to hemolysis either by 12 or 22 h homogenate. Determination of phospholipase C and sphingomyelinase activities showed that the hydrolysis rate of phospholipase C in homogenates prepared at 12–14 h was almost twice as much as sphingomyelinase activity. Whereas hydrolysis rates in 22–24 h homogenate were not different and markedly reduced compared to the 12–14 h homogenate. The times required for erythrocyte hemolysis related to the phospholipase C and sphingomyelinase activity profiles suggests that these enzyme activities participate in the in vitro hemolysis of red blood cells. Bovine and human erythrocytes change their biconcave contour into a spiculated spherical shape when they are exposed to midgut homogenate. This shape change is interpreted as a detergent induced modification of the red cell membrane which renders the erythrocytes more vulnerable to hemolysis.

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