重组蒙氏假单胞菌甲硫氨酸亚砜还原酶对外消旋亚砜的不对称还原分解

Q2 Chemical Engineering

Journal of Molecular Catalysis B-enzymatic Pub Date : 2016-11-01 DOI:10.1016/j.molcatb.2017.02.005

Jiawei Yang , Zhimei Yuan , Yang Zhou , Jia Zhao , Min Yang , Xiaoling Cheng , Gangwei Ou , Yongzheng Chen

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引用次数: 8

摘要

本研究报道了蒙氏假单胞菌(pseudomonas monteilii)菌株(pmMsrA)的蛋氨酸亚砜还原酶A通过不对称生物催化还原分解合成光学活性亚砜(R)-1a-4a。优化了几种生物转化参数，包括反应时间、细胞密度和底物浓度。此外，研究了pmMsrA催化不对称还原分解的底物范围，得到了手性(R)-1a-4a, ee为61% ~ 97%。

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Asymmetric reductive resolution of racemic sulfoxides by recombinant methionine sulfoxide reductase from a pseudomonas monteilii strain

In this study, the methionine sulfoxide reductase A from a pseudomonas monteilii strain (pmMsrA) was reported to synthesize optically active sulfoxides (R)-1a-4a, through asymmetric biocatalytic reductive resolution. Several biotransformation parameters including the reaction time, cell density, and substrate concentration were optimized. Moreover, Substrate scope of pmMsrA catalyzed asymmetric reductive resolution was investigated, which gave chiral (R)-1a-4a with 61%-97% ee.

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来源期刊

Journal of Molecular Catalysis B-enzymatic 生物-生化与分子生物学

CiteScore

2.58

自引率

0.00%

发文量

审稿时长

3.4 months

期刊介绍： Journal of Molecular Catalysis B: Enzymatic is an international forum for researchers and product developers in the applications of whole-cell and cell-free enzymes as catalysts in organic synthesis. Emphasis is on mechanistic and synthetic aspects of the biocatalytic transformation. Papers should report novel and significant advances in one or more of the following topics; Applied and fundamental studies of enzymes used for biocatalysis; Industrial applications of enzymatic processes, e.g. in fine chemical synthesis; Chemo-, regio- and enantioselective transformations; Screening for biocatalysts; Integration of biocatalytic and chemical steps in organic syntheses; Novel biocatalysts, e.g. enzymes from extremophiles and catalytic antibodies; Enzyme immobilization and stabilization, particularly in non-conventional media; Bioprocess engineering aspects, e.g. membrane bioreactors; Improvement of catalytic performance of enzymes, e.g. by protein engineering or chemical modification; Structural studies, including computer simulation, relating to substrate specificity and reaction selectivity; Biomimetic studies related to enzymatic transformations.