芒果花和仁提取物的化学成分、抗氧化和抗沙门氏菌活性

V. Kamble, Dipali O. Somkuwar, S. J. Wankhade
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引用次数: 9

摘要

研究了芒果花和种子部位提取物的抗氧化和抗沙门氏菌活性。采用圆盘扩散法和肉汤稀释法测定其抗沙门氏菌活性。采用DPPH法测定芒果花和果仁中石油醚、氯仿、丙酮和乙醇提取物的抗氧化活性,采用ICP-OES法测定芒果不同部位的矿物质含量。籽粒和花是铁的丰富来源,铁含量分别为660.50和221.75µg / g干重。采用DPPH自由基清除法测定提取物的抗氧化活性。花的石油醚提取物和乙醇提取物的抗沙门氏菌活性最显著,最低MIC值为8 mg/ml。以石油醚种仁和氯仿花提取物的抗氧化活性最高,抑制率分别为86.64±2.49和79.80±1.28。在所有试验组合中,氯仿花和乙醇籽仁提取物与四环素对伤寒沙门氏菌的协同作用更大。采用薄层色谱法从石油醚籽仁和丙酮花提取物中分离出4种具有抗氧化和抗沙门氏菌双重性能的化合物。经GC-MS分析,丙酮花提取物中含有1,2 -苯二甲酸异癸酯(邻苯二甲酸)。在石油醚种子仁提取物中发现了油酸和二十烷酯类化合物。本研究表明,所鉴定的化合物在花和种仁中具有抗氧化和抗沙门氏菌活性。这些发现可能证明芒果在治疗伤寒的常规使用。Kamble等,国际药学杂志,2016年9月,5(10):82-93 http://www.icpjonline.com/documents/Vol5Issue10/02.pdf
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemical composition, antioxidant and antisalmonella activity of Mangifera indica L. flower and seed kernel extracts
The extracts obtained from flower and seed parts of Mangifera indica L. were investigated for antioxidant and antisalmonella activities. Disc diffusion assay and broth macrodilution method were used to determine the antisalmonella activity. However, DPPH assay used to determine antioxidant activities of petroleum ether, chloroform, acetone and ethanol extracts of flower and seed kernel of Mangifera indica L. The mineral content of different parts of Mangifera indica L. was determined using ICP-OES method. Seed kernel and flower were found to be the rich source of Fe with the concentration of 660.50 and 221.75 µg per gram of dry weight. The antioxidant activity of the extracts was determined by DPPH free radical scavenging method. The petroleum ether extract of flower and ethanol extract of flower exhibited the most significant antisalmonella activity with the lowest MIC value of 8 mg/ml. The highest antioxidant activity was recorded in petroleum ether seed kernel and chloroform flower extract with the percentage inhibition of 86.64 ± 2.49 and 79.80 ± 1.28, respectively. Among all the tested combinations chloroform flower and ethanol seed kernel extracts showed greater synergistic effect along with tetracycline against Salmonella typhi isolates. Four different compounds with dual property of antioxidant and antisalmonella were isolated from the petroleum ether seed kernel and acetone flower extracts using thin layer chromatography. GC-MS analysis of acetonic flower extract showed the presence of 1, 2-Benzenedicarboxylic acid, isodecyl octyl ester (Synonym: Phthalic acid). Compounds Oleic acid and eicosyl ester were found in petroleum ether seed kernel extract. The present study revealed that identified compounds are responsible for exhibiting antioxidant and antisalmonella activities in flower and seed kernel. These findings may justify the conventional use of Mangifera indica L. in the treatment of typhoid fever. Kamble et al., International Current Pharmaceutical Journal, September 2016, 5(10): 82-93 http://www.icpjonline.com/documents/Vol5Issue10/02.pdf
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