PCR扩增日本血吸虫Sj26、Sj32和Sj14-3-3编码基因的诊断价值

Q3 Medicine

中华地方病学杂志 Pub Date : 2011-07-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.04.003

Shi-fei Cai, Li Wengui, Min Wang

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引用次数: 0

摘要

目的探讨Sj26、Sj32和Sj14-3-3编码基因PCR扩增对慢性日本血吸虫病的诊断价值。方法从40例慢性日本血吸虫病患者血清中提取DNA, PCR扩增Sj26、Sj32和Sj14-3-3编码基因，1.2%琼脂糖凝胶电泳鉴定。以21例华支睾吸虫病患者、13例威氏疏殖吸虫病患者和43例健康供者的血清DNA为对照。结果从日本血吸虫病患者血清中成功扩增出Sj14-3-3编码基因399 bp，但未扩增到Sj26(676 bp)和Sj32(1270 bp)编码基因。对照组均为阴性。结论PCR扩增的Sj14-3-3编码基因可用于慢性血吸虫病的遗传诊断。关键词:日本血吸虫病;聚合酶链反应;基因治疗

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Diagnostic value of Sj26, Sj32 and Sj14-3-3 coding gene of Schistosoma japonicum amplified by PCR

Objective To investigate the diagnostic value of coding gene of Sj26, Sj32 and Sj14-3-3 amplified by PCR for chronic Schistosomiasis japonica. Methods The DNA was extracted from sera of 40 patients with chronic Schistosomiasis japonica, the coding gene of Sj26, Sj32 and Sj14-3-3 was amplified by PCR and identified by 1.2% agarose gel electrophoresis. DNA from the sera of 21 patients with Clonorchiasis sinensis, 13 patients with Parogonimiasis westermani and 43 healthy donors was taken as control. Results A total of 399 bp coding gene of Sj14-3-3 was amplified successfully from sera of the patients with chronic Schistosomiasis japonica,but Sj26(676 bp) and Sj32( 1270 bp) coding gene were not obtained. Control groups were all negative. Conclusions Sj14-3-3 coding gene amplified by PCR can be used for genetic diagnosis of chronic schistosomiasis. Key words: Schistosomiasis japonica; Polymerase chain reaction; Gene therapy

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.