{"title":"剑麻废乙醇提取物的抗氧化活性及成分研究","authors":"Sidan Luo, Zhaomei Wang, Die Hu, Jian-yong Wu","doi":"10.17706/ijbbb.2019.9.4.248-257","DOIUrl":null,"url":null,"abstract":"Agave sisalana can be used as medicinal plant to treat some oxidative stress-caused diseases. This study aimed to fractionate and evaluate the antioxidant components of the ethanol extract from sisal waste. The crude ethanol extract of sisal waste was fractionated into petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (BF) and water fraction (WF). Among this different fractions, EAF had the strongest reducing power and radical scavenging capacity with IC50 of 40 mg/mL against DPPH· and 870 mg/mL against OH·, respectively, comparable to or even lower than that of BHT. EAF also had the highest content of total phenolic (214.6 mgGAE/g) and total flavonoid (179.8 mgRE/g). HPLC-MS analysis revealed the major components of EAF were flavanols and flavanones compounds.","PeriodicalId":13816,"journal":{"name":"International Journal of Bioscience, Biochemistry and Bioinformatics","volume":"235 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antioxidant Activity and Components of the Ethanol Extract of Sisal Waste\",\"authors\":\"Sidan Luo, Zhaomei Wang, Die Hu, Jian-yong Wu\",\"doi\":\"10.17706/ijbbb.2019.9.4.248-257\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Agave sisalana can be used as medicinal plant to treat some oxidative stress-caused diseases. This study aimed to fractionate and evaluate the antioxidant components of the ethanol extract from sisal waste. The crude ethanol extract of sisal waste was fractionated into petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (BF) and water fraction (WF). Among this different fractions, EAF had the strongest reducing power and radical scavenging capacity with IC50 of 40 mg/mL against DPPH· and 870 mg/mL against OH·, respectively, comparable to or even lower than that of BHT. EAF also had the highest content of total phenolic (214.6 mgGAE/g) and total flavonoid (179.8 mgRE/g). HPLC-MS analysis revealed the major components of EAF were flavanols and flavanones compounds.\",\"PeriodicalId\":13816,\"journal\":{\"name\":\"International Journal of Bioscience, Biochemistry and Bioinformatics\",\"volume\":\"235 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Bioscience, Biochemistry and Bioinformatics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17706/ijbbb.2019.9.4.248-257\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Bioscience, Biochemistry and Bioinformatics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17706/ijbbb.2019.9.4.248-257","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Antioxidant Activity and Components of the Ethanol Extract of Sisal Waste
Agave sisalana can be used as medicinal plant to treat some oxidative stress-caused diseases. This study aimed to fractionate and evaluate the antioxidant components of the ethanol extract from sisal waste. The crude ethanol extract of sisal waste was fractionated into petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (BF) and water fraction (WF). Among this different fractions, EAF had the strongest reducing power and radical scavenging capacity with IC50 of 40 mg/mL against DPPH· and 870 mg/mL against OH·, respectively, comparable to or even lower than that of BHT. EAF also had the highest content of total phenolic (214.6 mgGAE/g) and total flavonoid (179.8 mgRE/g). HPLC-MS analysis revealed the major components of EAF were flavanols and flavanones compounds.
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