自主跑轮运动对高脂饮食大鼠肺组织自噬状态的影响

IF 0.6 Q3 VETERINARY SCIENCES

Comparative Exercise Physiology Pub Date : 2023-03-17 DOI:10.3920/cep210022

J. Rezaie, A. Aboulhassani, R. Keyhanmanesh, R. Rahbarghazi, A. Delkhosh, L. Salimi, A. Zamani, A. Rahbarghazi, M. Ahmadi, F. Ghiasi

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引用次数: 0

摘要

本实验旨在探讨自主跑轮运动通过调节自噬反应对高脂饮食大鼠肺组织损伤的治疗作用。32只大鼠分为4组;正常饮食(对照组);VWR;高脂饮食(HFD)和HFD + VWR。3个月后观察HFD对肺组织的病理影响。检测支气管肺泡灌洗液(BALF)中肿瘤坏死因子(TNF)-α水平。我们监测了肺组织中白细胞介素(IL)-6和自噬相关基因的表达。H&E染色显示，与正常大鼠相比，HFD组的病理改变与支气管肺泡液中TNF-α水平升高一致。我们的结果显示，与对照组相比，HFD组IL-6、becline-1、LC3和P62表达上调。VWR可以抑制hfd诱导的变化，并通过调节自噬状态来降低hfd诱导的变化。

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Effect of voluntary wheel running on autophagy status in lung tissue of high-fat diet-fed rats

Here, we aimed to explore the therapeutic effect of voluntary wheel running (VWR) in high-fat diet-fed rats on pulmonary tissue injury via the modulation of autophagic response. Thirty-two rats were allocated into four groups; normal diet (Control); VWR; high-fat-diet (HFD), and HFD + VWR. After three months, pathological effect of HFD on pulmonary tissue was investigated. The levels of tumour necrosis factor (TNF)-α were detected in the bronchoalveolar lavage fluid (BALF). We monitored the expression of interleukin (IL)-6 and autophagy-related genes in lung tissues. H&E staining showed pathological changes in HFD group coincided with the increase of TNF-α levels in the bronchoalveolar fluid compared to the normal rats. Our results showed the up-regulation of IL-6, becline-1, LC3 and P62 in the HFD group compared to the Control group. VWR inhibited HFD-induced changes and could decrease HFD-induced changes via the regulation of autophagy status.

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来源期刊

Comparative Exercise Physiology VETERINARY SCIENCES-

CiteScore

1.50

自引率

11.10%

发文量

期刊介绍： ''Comparative Exercise Physiology'' is the only international peer-reviewed scientific journal specifically dealing with the latest research in exercise physiology across all animal species, including humans. The major objective of the journal is to use this comparative approach to better understand the physiological, nutritional, and biochemical parameters that determine levels of performance and athletic achievement. Core subjects include exercise physiology, biomechanics, gait (including the effect of riders in equestrian sport), nutrition and biochemistry, injury and rehabilitation, psychology and behaviour, and breeding and genetics. This comparative and integrative approach to exercise science ultimately highlights the similarities as well as the differences between humans, horses, dogs, and other athletic or non-athletic species during exercise. The result is a unique forum for new information that serves as a resource for all who want to understand the physiological challenges with exercise.