使用DVD拾取头检测单个生物细胞

TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference Pub Date : 2007-06-10 DOI:10.1109/SENSOR.2007.4300585

S. Kostner, M. Vellekoop

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引用次数: 7

摘要

在本文中，我们提出了一个光学检测系统的单个生物细胞，利用一个标准的DVD拾取头。在一项可行性研究中，我们展示了对悬浮在铂镜上的不同普通和镀银聚苯乙烯颗粒和细胞(酵母)的检测。在这些实验中，我们使用了拾音器的集成磁性actor来模拟粒子的运动，我们发现了显著的灵敏度。在此基础上，设计了一种具有集成反射镜的流动池，使其能够在细胞分析装置中测量颗粒。首先给出的测量结果表明，聚苯乙烯珠的灵敏度高，重复性好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Detection of Single Biological Cells using a DVD Pickup Head

In this paper we present an optical detection system for single biological cells that utilizes a standard DVD pickup head. In a feasibility study we have shown the detection for different plain and silver coated polystyrene particles and cells (yeast) suspended on a platinum mirror. For these experiments the integrated magnetic actor of the pickup was used to simulate the particle movement and we found a remarkable sensitivity. Then a flow cell with integrated mirror has been designed to be able to measure particles in a cytometric setup. First measurement results are given that show the high sensitivity and a good repeatability for polystyrene beads.

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TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference

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