Platelet Rich Fibrin (PRF) : un nouveau biomatériau de cicatrisation

The platelet rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified modes of production and without biochemical handling of blood (heparin, EDTA, bovine thrombin, calcium chloride…). In this second article, we were interested in the platelet associated features of this biomaterial. Indeed, during the production of the PRF by centrifugation, the blood platelets are activated, which induces their massive degranulation and the release of the platelet cytokines. The function of these molecules is crucial during first stages of any phenomenon of hemostasis and wound healing. The concentrations in cytokines obtained during the production of cPRP are already well known, whatever the protocol used. We thus undertook to measure out the concentrations of these molecules within the different parts of a PRF and the comparison between our data and those from some cPRP protocols. Our results revealed that the slow fibrin polymerization during the production of PRF would allow the intrinsic incorporation of the platelet cytokines in the fibrin meshing, especially in the network of glycanic chains trapped within the fibrin fibrillae. This result would imply that the PRF, unlike the other platelet concentrates, would be able to release progressively these cytokines with the degradation of the fibrin lattice: such a mechanism would explain the clinically observed healing properties of the PRF.