危害草莓品种的葡萄孢菌的研究

Altynai Bigazinovna Ismagulova, Z. Tulegenova, A. Spanbayev, Aida Shalabayeva
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引用次数: 0

摘要

灰腐真菌是一种危害草莓果实的有害病原菌,对其致病因子进行了研究。本研究工作的目的:分离出番荔枝(Fragaria moschata)有害病原菌的病原菌灰霉病菌(Botrytis cinerea),并对其进行全面研究。即通过分离真菌培养物和分子遗传学方法鉴定病原,再接种确定病原的致病性。灰腐病不仅在草莓生长季节,而且在收获后对草莓植株造成巨大的危害,使产品质量恶化。因此,全面研究和鉴定有害真菌病原体将为预防该病和组织防治措施提供实际依据。从受损草莓果实中分离出累积培养物和纯培养物,并通过形态培养和分子遗传学方法进行鉴定,结果表明99.62%的菌株属于葡萄孢杆菌。在草莓果实中接种证实了致病性。对我国草莓田造成严重危害的病原菌灰霉病菌(Botrytis cinerea)已通过现代分子和龙胆方法得到鉴定,并为组织进一步防治措施奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of the fungus Botrytis cinerea that damages strawberry species
The paper studies the causative agent of fungal gray rot, a harmful pathogen that affects the fruits of strawberry species that are in high demand and berries of which have beneficial properties. The purpose of the research work: to isolate the fungus Botrytis cinerea, the causative agent of the harmful pathogen of the fruit Fragaria moschata, and to study it comprehensively. That is, to identify the pathogen by separating fungal cultures and identifying it by molecular-genetic method and determining its pathogenicity by re-inoculation. The disease of gray rot causes enormous damage to the strawberry plant not only during the growing season, but also after harvesting, worsening the quality of the product. Therefore, a comprehensive study and identification of harmful fungal pathogens will serve as a practical basis for preventing the disease and organizing measures to combat it. Isolation of accumulative and pure cultures by growing in a solid nutrient medium from damaged strawberry fruits and identification by morphological-cultural and molecular-genetic methods, as a result of the analysis, 99.62 % corresponded to the species Botrytis cinerea. Pathogenicity is proven by inoculation in strawberry fruits. The pathogen fungus Botrytis cinerea, which causes significant damage to Strawberry Fields in the country, has been identified by modern molecular and gentian methods and serves as the basis for organizing further measures to combat it.
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