不同条件下酿酒酵母生长参数的特点

Anahit H. Shirvanyan, Satenik N. Mirzoyan, K. Trchounian
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引用次数: 0

摘要

酿酒酵母广泛应用于食品工业,是人类文明的重要组成部分。在工业应用中,获得大量的生物质是非常重要的,但导致酵母活力丧失和死亡的因素很多。本文研究了酿酒酵母葡萄酒和啤酒菌株在不同外部条件下生长参数-特定生长率(SGR, μ,生长量)的特点,以及生长过程中pH和氧化还原电位(ORP)的变化。结果表明,在好氧培养条件下,30℃和pH 6.5更有利于2株菌株(ATCC 9804, μ = 0.398;ATCC 13007, μ = 0.407),在微氧条件下pH 5.0和37℃更有利(ATCC 9804, μ = 0.35;atc13007, μ = 0.436)。在好氧条件下,建立了较低的ORP值,该值不依赖于生长温度和外部pH。在微氧条件下,直到固定相建立(24 h), ORP的pH依赖性变化已经建立。所得结果将有助于阐明酵母生存的生化、生物物理和生物能量现象,从而提高生物量增长和产量,从而实现工业规模应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PECULIARITIES OF GROWTH PARAMETERS OF $SACCHAROMYCES~CEREVISIAE$ UNDER DIFFERENT CONDITIONS
Saccharomyces cerevisiae is an essential component of human civilization because of its extensive use in food industry. At the point of industrial usage it is very important to get large amount of biomass but many factors causes yeast's viability loss and death. The aim of this work was to study the peculiarities of growth parameters – specific growth rate (SGR, μ, growth yield) of S. cerevisiae wine and beer strains under different external conditions, as well as pH and oxidation-reduction potential (ORP) changes during growth. It was shown that under aerobic cultivation, the conditions 30℃ and pH 6.5 are more favorable for the growth of 2 strains (ATCC 9804, μ = 0.398; ATCC 13007, μ = 0.407), where they can enhance the biomass, and under microaerobic conditions the pH 5.0 and 37℃ are more favorable (ATCC 9804, μ = 0.35; ATCC 13007, μ = 0.436). During aerobic conditions, lower ORP values are established which did not depend on growth temperature and external pH. Under microaerobic conditions until the establish¬ment of the stationary phase (24 h) pH dependent changes of ORP were established. Obtained results will help to clarify the biochemical, biophysical and bioenergetic phenomena of yeast survival that can enhance the biomass growth and yield and thus applied industrial scale.
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