紫贻贝中与粘附有关的酚氧化酶的研究

Agricultural and biological chemistry Pub Date : 1991-11-23 DOI:10.1271/BBB1961.55.2887

Naohiro Maruyama, H. Etoh, K. Sakata, K. Ina

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引用次数: 9

摘要

以左旋多巴为底物测定酪氨酸酶活性。用Pomerantz12法对酶活性进行了轻微的修饰，分光光度法测定酶活性。酶液(0.2ml)与1.5ml 4mm D,L-dopa在0.1m磷酸钠缓冲液中孵育，pH为6.5，含0.9% (w/v) NaCl, 25℃下孵育。观察左旋多巴向多巴色的转化，观察吸光度的增加

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Studies on phenoloxidase from Mytilus edulis associated with adhesion

Assay for tyrosinase activity using L-dopa as the substrate. The enzyme activity was measured spectrophotometrically by the method of Pomerantz12) with a slight modification. The enzyme solution (0.2ml) was incubated at 25°C with 1.5ml of4mM D,L-dopa in 0.1m sodium phosphate buffer, pH 6.5, containing 0.9% (w/v) NaCl. The conversion of L-dopa to dopachrome was followed by monitoring the increase of absorbance at

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Agricultural and biological chemistry

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