棉花Rho家族的全基因组特征提供了对纤维发育的见解

IF 3.1 4区 生物学 Q2 PLANT SCIENCES
Man He, Xingfen Wang, Shang Liu, Hailiang Cheng, Dongyun Zuo, Qiaolian Wang, Limin Lv, Youping Zhang, G. Song
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引用次数: 0

摘要

棉花是全球天然纤维的来源,满足了纺织业90%的需求。然而,纤维的发育是一个复杂的生物过程,包括四个阶段。纤维从单个细胞发育而来,细胞伸长是纤维发育的重要过程。因此,了解和开发纤维发育过程中细胞伸长的机制是相关的。之前关于真核生物细胞分裂控制蛋白42 (CDC-42)在细胞伸长中的关键作用的报道启发我们探索其同源Rho GTPases,以了解棉纤维发育过程中的细胞伸长。结果8种棉属植物的2066个Rho蛋白分别在A亚基因组和D亚基因组中分为5个和8个类群。在5个四倍体中观察到Rho成员的不对称进化。两种短纤维和长纤维基因型的群体固定统计发现,水蛭中存在34个Rho基因的高度分化区域,其中31个Rho基因通过基因组全关联分析(GWAS)进一步验证。此外,基于先前发表的转录组数据,加权基因共表达网络表征了Rho基因的全基因组表达模式。从5个模块中鉴定出20个Rho基因为枢纽基因,可能与纤维发育有关。基于转录丰度和基因本体(GO)富集的5个Rho基因相互作用网络强调了Rho参与细胞壁生物合成、脂肪酸延伸和其他生物过程。结论本研究表征了棉花中的Rho蛋白,为棉纤维的细胞伸长及棉纤维改良提供了新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-wide characterization of the Rho family in cotton provides insights into fiber development
Background Cotton is the source of natural fibers globally, fulfilling 90% of the textile industry's requirements. However, fiber development is a complex biological process comprising four stages. Fiber develops from a single cell, and cell elongation is a vital process in fiber development. Therefore, it is pertinent to understand and exploit mechanisms underlying cell elongation during fiber development. A previous report about cell division control protein 42 (CDC-42) with its key role in cell elongation in eukaryotes inspired us to explore its homologs Rho GTPases for understanding of cell elongation during cotton fiber development. Result We classified 2 066 Rho proteins from 8 Gossypium species into 5 and 8 groups within A and D sub-genomes, respectively. Asymmetric evolution of Rho members was observed among five tetraploids. Population fixation statistics between two short and long fiber genotypes identified highly diverged regions encompassing 34 Rho genes in G. hirustum, and 31 of them were retained through further validation by genome wide association analysis (GWAS). Moreover, a weighted gene co-expression network characterized genome-wide expression patteren of Rho genes based on previously published transcriptome data. Twenty Rho genes from five modules were identified as hub genes which were potentially related to fiber development. Interaction networks of 5 Rho genes based on transcriptional abundance and gene ontology (GO) enrichment emphasized the involvement of Rho in cell wall biosynthesis, fatty acid elongation, and other biological processes. Conclusion Our study characterized the Rho proteins in cotton, provided insights into the cell elongation of cotton fiber and potential application in cotton fiber improvement.
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来源期刊
Journal of Cotton Research
Journal of Cotton Research PLANT SCIENCES-
CiteScore
5.20
自引率
3.80%
发文量
99
审稿时长
15 weeks
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