利用光热纳米叶片直接核递送DNA大分子

2013 International Conference on Optical MEMS and Nanophotonics (OMN) Pub Date : 2013-11-11 DOI:10.1109/OMN.2013.6659067

Ting-Hsiang Wu, Yi-Chien Wu, Enrico Sagullo, M. Teitell, P. Chiou

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引用次数: 0

摘要

我们演示了使用光热纳米叶片将大分子直接核递送到活的哺乳动物细胞中。利用脉冲激光触发钛薄膜微移管尖端的空化气泡，刺穿细胞质和核膜，然后通过压力控制将货物输送到细胞核。质粒在不同类型的细胞中高表达(79-100%)，并保持细胞活力。

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Direct nuclear delivery of DNA macromolecules using the photothermal nanoblade

We demonstrated direct nuclear delivery of macromolecules into live mammalian cells using the photothermal nanoblade. Pulsed laser triggered cavitation bubbles on a titanium thin film coated micropipette tip was utilized to puncture both the cell plasma and nuclear membranes followed by pressure controlled delivery of cargo into the nucleus. High plasmid expression (79-100%) in different cell types with maintained cell viability was achieved.

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2013 International Conference on Optical MEMS and Nanophotonics (OMN)

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