低氧性肺动脉高压大鼠模型肺动脉蛋白质组学分析

IF 0.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Current Proteomics Pub Date : 2022-02-04 DOI:10.2174/1570164619666220204123709

Ma Shuang, L. Jie, Zhang Ruixia, Liu Chuanchuan, M. Yan

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引用次数: 0

摘要

背景:低氧性肺动脉高压(HPH)是一种以高海拔地区肺动脉压力持续升高为特征的病理状态。目的:探讨SD大鼠缺氧4周后的蛋白质组学特征及其反应机制。方法:对低氧性肺动脉高压大鼠模型肺动脉进行蛋白质组学分析。结果:共鉴定出3204个蛋白，其中上调49个，下调46个。上调的基因包括Prolargin、Protein S100-A6和Transgelin-2，而Nascent polypeptide associated complex和Elongator complex Protein 1下调。低氧组KEGG富集通路对嘌呤代谢、肿瘤和脂肪分解具有显著的调节作用。结论:本研究为后续组织缺氧机制及相关生理条件的研究提供了基础。低氧性肺动脉高压(HPH)是一种病理状况，以高海拔地区肺动脉压持续升高为例。在此，我们研究了Sprague-Dawley (SD)大鼠在4周的缺氧条件下的蛋白质组谱和反应机制。基于无偏itraq的定量蛋白质组学应用于暴露于HPH的大鼠模型的蛋白质组学分析。在鉴定的3204个蛋白中，49个蛋白上调，46个蛋白下调。上调的基因包括Prolargin、Protein、S100-A6和Transgelin-2，而Nascent polypeptide associated complex和Elongator complex Protein 1则下调。京都基因与基因组百科全书(KEGG)富集通路具有嘌呤代谢、癌症和脂肪分解调节，在缺氧组显著富集。总之，本研究的发现为组织缺氧机制及相关生理条件的下游研究提供了基础。

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Proteomic profile analysis of pulmonary artery in a rat model under hypoxic pulmonary hypertensionc

Proteomic profile analysis of pulmonary artery in a rat model under hypoxic pulmonary hypertension Background: Hypoxic pulmonary hypertension (HPH) is a pathological condition exemplified by a constant rise in pulmonary artery pressure in high-altitudes. Objective: To investigated the proteome profile and response mechanisms of SD rats under hypoxia over a period of four-weeks. Method: Proteomic profile analysis of pulmonary artery in a rat model under hypoxic pulmonary hypertension. Results: With 3,204 proteins identified, 49 were up-regulated while 46 were down-regulated. Upregulated genes included Prolargin, Protein S100-A6 and Transgelin-2, whereas Nascent polypeptide-associated complex and Elongator complex protein 1 were down-regulated. KEGG enriched pathways had purine metabolism, cancer and lipolysis regulation as significantly enriched in hypoxic group. Conclusion: In conclusion, our findings submit basis for downstream studies on tissue hypoxia mechanisms alongside the associated physiological conditions. Hypoxic pulmonary hypertension (HPH) is a pathological condition exemplified by a constant rise in pulmonary artery pressure in high altitudes. Herein, we investigated the proteome profile and response mechanisms of Sprague-Dawley (SD) rats under hypoxia over a period of four weeks. Unbiased iTRAQ-based quantitative proteomics was utilized in proteome profile analysis of a rat model exposed to HPH. With 3,204 proteins identified, 49 were upregulated while 46 were downregulated. Upregulated genes included Prolargin, Protein, S100-A6 and Transgelin-2, whereas Nascent polypeptide-associated complex and Elongator complex protein 1 were downregulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enriched pathways had purine metabolism, cancer, and lipolysis regulation as significantly enriched in hypoxic group. In conclusion, the findings from this study submit a basis for downstream studies on tissue hypoxia mechanisms alongside the associated physiological conditions.

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来源期刊

Current Proteomics BIOCHEMICAL RESEARCH METHODS-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed in-depth/mini review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry. Current Proteomics publishes in-depth/mini review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, software, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to: Protein separation and characterization techniques 2-D gel electrophoresis and image analysis Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching Determination of co-translational and post- translational modification of proteins Protein/peptide microarrays Biomolecular interaction analysis Analysis of protein complexes Yeast two-hybrid projects Protein-protein interaction (protein interactome) pathways and cell signaling networks Systems biology Proteome informatics (bioinformatics) Knowledge integration and management tools High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography) High-throughput computational methods for protein 3-D structure as well as function determination Robotics, nanotechnology, and microfluidics.