Antioxidant and inflammatory cytokines regulatory actions of fresh snail and seawater gastropods extracts

Context: Mollusca also termed mollusks. These are unsegmented, bilateral animals. Freshwater and seawater gastropods are considered rich source of bioactive molecules that possesses various therapeutic potential. Aim: The present work aimed to investigate the antioxidant, wound healing, and anti-inflammatory effects along with the regulation of inflammatory protein at the cellular level by apple snail (freshwater gastropod) and tibia shell (seawater gastropod) extract. Materials and Methods: The mass of freshwater and seawater gastropods was macerated in water. Results: The aqueous extract of apple snail exhibited the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and 2,2'-azinobis-(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) activity at 85.62% and 73.35% at 10 mg/ml, whereas the aqueous extract of seawater gastropod exhibited the highest DPPH radical scavenging activity and ABTS activity 83.53% and 76.14% at 1 mg/ml. Marine water snail aqueous extract and freshwater snail aqueous extract, each of 250 mg/kg showed 83% and 67.14% inhibition of paw edema, respectively. The cell viability study was performed by MTT assay and both the extracts showed cell viability above 97%. Both samples (freshwater snail extract and seawater gastropod extract) have good anti-inflammatory effects as they lower the concentration of tumor necrosis factor-alpha (pro-inflammatory cytokines) and increase interleukin-10 (anti-inflammatory cytokines). Conclusion: The present work concluded that the extracts of seawater gastropods were found to be more effective as an antioxidant and anti-inflammatory agent.