生物场处理对酪蛋白酶水解物和酪蛋白酵母蛋白胨物理和热性质影响的评价

M. Trivedi, G. Nayak, S. Patil, R. M. Tallapragada, S. Jana, R. Mishra
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引用次数: 5

摘要

本研究研究了生物场处理对酪蛋白水解酶(CEH)和酪蛋白酵母蛋白胨(CYP)物理和热性质的影响。采用傅里叶红外光谱(FT-IR)、差示扫描量热法(DSC)、热重分析(TGA)、粒度和表面积分析对对照和处理后的样品进行了表征。FTIR结果表明,与对照组相比,生物场处理导致了CEH中酰胺基团(酰胺i和酰胺ii)拉伸振动峰的减少,这与分子间氢键的增强有关。然而,CYP处理后的FTIR光谱未见明显变化。TGA分析表明,处理后的CEH在热稳定性方面有了很大的改善,与对照(209°C)相比,最高热分解温度(217°C)有所提高。同样,与对照组相比,处理过的CYP也表现出更高的热稳定性。DSC显示处理后的CYP的熔化温度比对照组升高。然而,处理后的CEH在DSC中没有出现熔化峰,这可能是由于蛋白质链的刚性所致。与对照相比,处理后的CEH表面积增加了83%。然而,CYP处理后,表面面积减少(7.3%)。经处理的CEH的粒径分析显示,与对照样品相比,平均粒径(d50)和d99值(存在99%颗粒的最大粒径)显着增加。同样,处理过的CYP也显示出d50和d99值的大幅增加,这可能是由于颗粒聚集导致形成更大的微粒。结果表明,经生物场处理后的CEH和CYP可作为制药应用的基质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the Impact of Biofield Treatment on Physical and Thermal Properties of Casein Enzyme Hydrolysate and Casein Yeast Peptone
In the present study, the influence of biofield treatment on physical and thermal properties of Casein Enzyme Hydrolysate (CEH) and Casein Yeast Peptone (CYP) were investigated. The control and treated samples were characterized by Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), Thermo Gravimetric Analysis (TGA), particle size and surface area analysis. The FTIR results revealed that biofield treatment has caused reduction of amide group (amide-I and amide-II) stretching vibration peak that is associated with strong intermolecular hydrogen bonding in treated CEH as compared to control. However, no significant changes were observed in FTIR spectrum of treated CYP. The TGA analysis of treated CEH showed a substantial improvement in thermal stability which was confirmed by increase in maximum thermal decomposition temperature (217°C) as compared to control (209°C). Similarly, the treated CYP also showed enhanced thermal stability as compared to control. DSC showed increase in melting temperature of treated CYP as compared to control. However the melting peak was absent in DSC of treated CEH which was probably due to rigid chain of the protein. The surface area of treated CEH was increased by 83% as compared to control. However, a decrease (7.3%) in surface area was observed in treated CYP. The particle size analysis of treated CEH showed a significant increase in average particle size (d50) and d99 value (maximum particle size below which 99% of particles are present) as compared to control sample. Similarly, the treated CYP also showed a substantial increase in d50 and d99 values which was probably due to the agglomeration of the particles which led to formation of bigger microparticles. The result showed that the biofield treated CEH and CYP could be used as a matrix for pharmaceutical applications.
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