正畸带对人牙龈成纤维细胞氧化应激反应的毒性评价。

Alexandre Marcos Bandeira, E. F. Martinez, A. Demasi
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引用次数: 8

摘要

目的探讨不锈钢正畸带的细胞毒性及其对人牙龈成纤维细胞抗氧化基因表达的影响。材料与方法每个品牌(Dentsply-Sirona、Dentaurum、TP Orthodontics和Morelli)的条带在0.2 g/mL培养液中37°C条件下培养14 d,将相应的条件培养基涂于成纤维细胞上。通过台盼蓝排斥试验,在条件培养基中暴露24、48和72小时后评估细胞活力。定量聚合酶链反应测定暴露24 h后抗氧化防御基因过氧化物还蛋白1 (PRDX1)、超氧化物歧化酶1 (SOD1)和谷胱甘肽过氧化物酶1 (GPX1)的表达。将这些参数与未暴露于条带条件培养基(对照组)的细胞的参数进行比较。结果在48和72 h时,各条带均能促进活细胞数的减少(P < 0.01)。基因表达分析显示,Dentsply-Sirona、TP Orthodontics和Morelli带的条件培养基导致PRDX1转录本水平显著升高(P < 0.01), Dentaurum和Morelli带的条件培养基诱导SOD1水平显著升高(P < 0.01)。GPX1的表达不受条件培养基的影响。结论正畸带对成纤维细胞具有一定的毒性,可增加PRDX1和SOD1抗氧化基因的表达,诱导细胞氧化应激。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of toxicity and response to oxidative stress generated by orthodontic bands in human gingival fibroblasts.
OBJECTIVE To evaluate the cytotoxicity of stainless-steel orthodontic bands and their influence on the expression of the antioxidant genes in human gingival fibroblasts. MATERIALS AND METHODS Ten bands of each brand (Dentsply-Sirona, Dentaurum, TP Orthodontics, and Morelli) were conditioned in 0.2 g/mL culture medium at 37°C for 14 days, and the corresponding conditioned media were applied over the fibroblasts. Cell viability was assessed after 24, 48, and 72 hours of exposure to the conditioned media by trypan blue exclusion assay. Expression of the antioxidant defense genes peroxiredoxin 1 (PRDX1), superoxide dismutase 1 (SOD1), and glutathione peroxidase 1 (GPX1) were evaluated by quantitative polymerase chain reaction after 24 hours of exposure. These parameters were compared to those of the cells not exposed to the conditioned media of the bands (control). RESULTS All bands promoted a reduction in the number of viable cells in the periods of 48 and 72 hours (P < .01). Analysis of gene expression showed a significant increase in the levels of PRDX1 transcripts caused by the conditioned media of the Dentsply-Sirona, TP Orthodontics, and Morelli bands (P < .01) as well as induction of SOD1 by the conditioned media of the Dentaurum and Morelli (P < .01). Expression of GPX1 was not influenced by the conditioned media. CONCLUSIONS The orthodontic bands showed toxicity to fibroblasts and increased the expression of PRDX1 and SOD1 antioxidant genes, indicating induction of oxidative stress in the cells.
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