{"title":"粪肠球菌LR/6产细菌素的制备及特性研究","authors":"Manoj Kumar, N. Ghosh, S. Srivastava","doi":"10.5580/1530","DOIUrl":null,"url":null,"abstract":"A soil isolate of Lactic acid bacteria (LAB), strain LR/6 was identified as Enterococcus faecium on the basis of its morphological, biochemical, and 16S rDNA analysis. Strain LR/6 is a potential producer of an antimicrobial compound that followed the kinetic pattern typical of a primary metabolite synthesis and is released extracellularly. The antimicrobial action remained unaffected when treated with β-glycerophosphate and catalase suggesting that the activity is neither based on acid nor H2O2 production. This antimicrobial compound was highly stable over a wide range of pH (2.0 to 8.0), high temperature (boiling and autoclaving) and could be stored stably at a range of temperature (-20oC to 37oC) at least upto one year. The proteinaceous nature of the antimicrobial compound was ascertained by its sensitivity to many proteolytic enzymes confirming it to be a bacteriocin. Strain LR/6 bacteriocin was also insensitive to the presence of α-amylase, lipase, surfactants, organic solvents, and showed a bactericidal mode of action. Crude bacteriocin exhibited broad inhibition spectrum both against related as well as some foodborne pathogenic bacteria including Listeria monocytogenes. Tricine SDS-PAGE analysis and bacteriocin activity assay corresponded to a protein of an apparent molecular mass of ~6.0 kDa.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":"{\"title\":\"Production and characterization of a bacteriocin produced by Enterococcus faecium LR/6\",\"authors\":\"Manoj Kumar, N. Ghosh, S. Srivastava\",\"doi\":\"10.5580/1530\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A soil isolate of Lactic acid bacteria (LAB), strain LR/6 was identified as Enterococcus faecium on the basis of its morphological, biochemical, and 16S rDNA analysis. Strain LR/6 is a potential producer of an antimicrobial compound that followed the kinetic pattern typical of a primary metabolite synthesis and is released extracellularly. The antimicrobial action remained unaffected when treated with β-glycerophosphate and catalase suggesting that the activity is neither based on acid nor H2O2 production. This antimicrobial compound was highly stable over a wide range of pH (2.0 to 8.0), high temperature (boiling and autoclaving) and could be stored stably at a range of temperature (-20oC to 37oC) at least upto one year. The proteinaceous nature of the antimicrobial compound was ascertained by its sensitivity to many proteolytic enzymes confirming it to be a bacteriocin. Strain LR/6 bacteriocin was also insensitive to the presence of α-amylase, lipase, surfactants, organic solvents, and showed a bactericidal mode of action. Crude bacteriocin exhibited broad inhibition spectrum both against related as well as some foodborne pathogenic bacteria including Listeria monocytogenes. Tricine SDS-PAGE analysis and bacteriocin activity assay corresponded to a protein of an apparent molecular mass of ~6.0 kDa.\",\"PeriodicalId\":22514,\"journal\":{\"name\":\"The Internet journal of microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/1530\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/1530","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Production and characterization of a bacteriocin produced by Enterococcus faecium LR/6
A soil isolate of Lactic acid bacteria (LAB), strain LR/6 was identified as Enterococcus faecium on the basis of its morphological, biochemical, and 16S rDNA analysis. Strain LR/6 is a potential producer of an antimicrobial compound that followed the kinetic pattern typical of a primary metabolite synthesis and is released extracellularly. The antimicrobial action remained unaffected when treated with β-glycerophosphate and catalase suggesting that the activity is neither based on acid nor H2O2 production. This antimicrobial compound was highly stable over a wide range of pH (2.0 to 8.0), high temperature (boiling and autoclaving) and could be stored stably at a range of temperature (-20oC to 37oC) at least upto one year. The proteinaceous nature of the antimicrobial compound was ascertained by its sensitivity to many proteolytic enzymes confirming it to be a bacteriocin. Strain LR/6 bacteriocin was also insensitive to the presence of α-amylase, lipase, surfactants, organic solvents, and showed a bactericidal mode of action. Crude bacteriocin exhibited broad inhibition spectrum both against related as well as some foodborne pathogenic bacteria including Listeria monocytogenes. Tricine SDS-PAGE analysis and bacteriocin activity assay corresponded to a protein of an apparent molecular mass of ~6.0 kDa.