Anti-cancer Activity of Leaf Extract Preparation from Ipomoea sepiaria against PC-3 Cell Line

Researches on PC-3 human prostate cancer cell lines control are needed in the present decades. The anticancer activity of aqueous extract of Ipomoea sepiaria was investigated by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium (MTT) assay using PC-3 cell line. The present experimentation was showed that aqueous extract of Ipomoea sepiaria, when subjected to different concentrations on PC-3 cells showed IC50 cell inhibition at about 5μM for 48 hours and about 2μM for 72 hours. PTEN interacts with other expression proteins like Jun proto-oncogene, V-akt murine thymoma viral oncogene homolog 1 and 2, Tumor protein p53, Phosphatidylinositol-4,5-bisphosphate 3-kinase, etc. The present experiment shown that the leaf extract may involve in protein suppressor mechanism for PTEN in controlling of prostate cancer. Key-wordsPC-3, Ipomoea sepiaria, Anticancer activity, MTT assay, Prostate cancer INTRODUCTION One of the source plants of the classical herb Lakshmana is Ipomoea sepiaria Koenig Ex. Roxb belongs to the family Convolvulaceae. Ipomoea sepiaria (I. sepiaria), a perennial climber is an important ethanomedicinal plant having phyto-constituents like alkaloids, carbohydrates, flavonoids, glycosides, saponin, tannin and phenolic compounds. PC3 (PC-3) is a human prostate cancer cell lines that are highly used in investigating the biochemical changes prostatic cancer cells . From the last few years, Phytotherapy (herbal therapies) usage for prostate cancer has been increasing dramatically. Several herbs like Chrysanthemum morifolium , Ganoderma lucidum (a root fungus) , Glycyrrhiza glabra (Spanish liquorice) , Scutellaria baicalensis , Panax pseudoginseng , Dendranthema morifolium , Rabdosia rubescens , and Isatis indigotica [10] are tested effective in the treatment of PC-3 cell lines. There were no reports for using Ipomoea sepiaria as a medicinal plant that was used against PC-3 cell line for treating prostatic cancer. A candidate tumor suppressor gene, PTEN (putative protein tyrosine phosphatase) gene is considered as the responsibility of causing prostate cancer . Access this article online Quick Response Code Website: