双孢蘑菇核糖体RNA基因和分散重复DNA元件家族胞嘧啶甲基化的证据

Aimin Li, Paul A. Horgen
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引用次数: 5

摘要

Li, A.和Horgen, P. A. 1993。双孢蘑菇核糖体RNA基因和分散重复DNA元件家族中胞嘧啶甲基化的证据。实验真菌学,17,356-361。利用限制性内切酶分析和DNA杂交证实了蘑菇重复DNA序列的甲基化。我们的结果表明,5-甲基胞嘧啶(5mC)是唯一检测到的甲基化碱基。该核苷酸作为CCGG识别序列的内胞嘧啶存在于核糖体RNA基因中,也存在于商业蘑菇、双孢蘑菇(Agaricus bisporus)和其他蘑菇(Agaricus)的分散重复DNA元件家族中。密度分析显示rDNA的甲基化程度高于分散的重复元件。在线粒体核糖体RNA基因和双孢螺旋藻线粒体基因组的反向重复序列中均未检测到胞嘧啶残基的甲基化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evidence for Cytosine Methylation in Ribosomal RNA Genes and in a Family of Dispersed Repetitive DNA Elements in Agaricus bisporus and Selected Other Agaricus Species

Li, A., and Horgen, P. A. 1993. Evidence for cytosine methylation in ribosomal RNA genes and in a family of dispersed repetitive DNA elements in Agaricus bisporus and selected other Agaricus species. Experimental Mycology 17, 356-361. Evidence for the methylation of Agaricus repetitive DNA sequences was demonstrated using restriction enzyme analysis and DNA hybridization. Our results indicate that 5-methylcytosine (5mC) was the only methylated base detected. The nucleotide was present as the internal cytosine of the CCGG recognition sequence in the ribosomal RNA genes and in a family of dispersed repetitive DNA elements of the commercial mushroom, Agaricus bisporus and selected other Agaricus species. Densitometry analysis revealed more methylation in the rDNA than in the dispersed repetitive elements. No methylation of cytosine residues was detected in mitochondrial ribosomal RNA genes nor in the inverted repeated sequences of the A. bisporus mitochondrial genome.

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