Optimization of Ultrasound-assisted Extraction of Ulvan from Green Seaweed Ulva lactuca

In this paper, the extraction conditions of ulvan from green seaweed Ulva lactuca by using Ultrasound-assisted extraction method were optimised. Experience was designed using Box-behnken model with experient areas as following: extraction temperature (X1: 50–90 °C), extraction time (X2: 20–40, min) and solvent-to-material ratio (X3: 50/1–70/1, v/w), objective function (Y: extraction yield) was required to get maximum value. The result showed that the optimization conditions for extraction were: extraction temperature 84.28 °C, extraction time 30.59 min with 60.21/1 (v/w) as solvent-to-material ratio, and the expected objective function Y is 22.86% based on dried seaweed weight.