{"title":"对辣椒杆菌和葡萄球菌菌油的抗菌活性进行测试","authors":"Yurisa Yurisa, M. A. Wibowo","doi":"10.26418/indonesian.v2i2.36905","DOIUrl":null,"url":null,"abstract":"Essential oils of Jeruk Sambal leaves (Citrus x hystryx) have as antibacterial agents. This study aims to determine the antibacterial activity of dried Jeruk Sambal leaves originating from Sungai Kakap area against Escherichia coli and Staphylococcus aureus. Stages in this study were divided into two stages (1) isolation of volatile oil by steam distillation, (2) test the antibacterial activity by pitting diffusion method. Essential oil yield of 0.22% was obtained. The results of GC-MS analysis showed that the essential oil of Jeruk Sambal leaves dried has 4 main compounds, namely elemol (26.27%), β-eudesmol (14.47%), 2-β-pinene (13.84%) and linalool (7.77%). The test results showed that the antibacterial activity of essential oils of Jeruk Sambal leaves was more active in inhibiting the growth of S. aureus than E. coli bacteria.","PeriodicalId":13554,"journal":{"name":"Indonesian Journal of Pure and Applied Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN JERUK SAMBAL (Citrus x hystryx) TERHADAP Escherichia coli DAN Staphylococcus aureus\",\"authors\":\"Yurisa Yurisa, M. A. Wibowo\",\"doi\":\"10.26418/indonesian.v2i2.36905\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Essential oils of Jeruk Sambal leaves (Citrus x hystryx) have as antibacterial agents. This study aims to determine the antibacterial activity of dried Jeruk Sambal leaves originating from Sungai Kakap area against Escherichia coli and Staphylococcus aureus. Stages in this study were divided into two stages (1) isolation of volatile oil by steam distillation, (2) test the antibacterial activity by pitting diffusion method. Essential oil yield of 0.22% was obtained. The results of GC-MS analysis showed that the essential oil of Jeruk Sambal leaves dried has 4 main compounds, namely elemol (26.27%), β-eudesmol (14.47%), 2-β-pinene (13.84%) and linalool (7.77%). The test results showed that the antibacterial activity of essential oils of Jeruk Sambal leaves was more active in inhibiting the growth of S. aureus than E. coli bacteria.\",\"PeriodicalId\":13554,\"journal\":{\"name\":\"Indonesian Journal of Pure and Applied Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indonesian Journal of Pure and Applied Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26418/indonesian.v2i2.36905\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indonesian Journal of Pure and Applied Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26418/indonesian.v2i2.36905","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
柚子叶精油(Citrus x hystryx)有抗菌作用。本研究旨在测定产自Sungai Kakap地区的干参巴叶对大肠杆菌和金黄色葡萄球菌的抑菌活性。本研究分为两个阶段:(1)蒸汽蒸馏法分离挥发油，(2)点蚀扩散法检测抗菌活性。精油得率为0.22%。气相色谱-质谱分析结果表明，三叶精油中含有4种主要化合物，分别为香柠檬酚(26.27%)、β-苦艾酚(14.47%)、2-β-蒎烯(13.84%)和芳樟醇(7.77%)。实验结果表明，山参叶精油对金黄色葡萄球菌的抑菌活性比大肠杆菌更强。
UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN JERUK SAMBAL (Citrus x hystryx) TERHADAP Escherichia coli DAN Staphylococcus aureus
Essential oils of Jeruk Sambal leaves (Citrus x hystryx) have as antibacterial agents. This study aims to determine the antibacterial activity of dried Jeruk Sambal leaves originating from Sungai Kakap area against Escherichia coli and Staphylococcus aureus. Stages in this study were divided into two stages (1) isolation of volatile oil by steam distillation, (2) test the antibacterial activity by pitting diffusion method. Essential oil yield of 0.22% was obtained. The results of GC-MS analysis showed that the essential oil of Jeruk Sambal leaves dried has 4 main compounds, namely elemol (26.27%), β-eudesmol (14.47%), 2-β-pinene (13.84%) and linalool (7.77%). The test results showed that the antibacterial activity of essential oils of Jeruk Sambal leaves was more active in inhibiting the growth of S. aureus than E. coli bacteria.
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