{"title":"利用普通小麦和花生凝集素对黄颡鱼种群进行种内鉴定","authors":"J. Schottelius , G. Uhlenbruck","doi":"10.1016/S0174-3031(84)80012-8","DOIUrl":null,"url":null,"abstract":"<div><p>The agglutination behaviour of four-days-old epimastigote culture forms of <em>T. cruzi</em>, and <em>T. cruzi</em>-like stocks from different South American countries were tested with 15 lectins. Two agglutination types can be distinguished: Typ 1 includes stocks which react with <em>Triticum vulgaris</em> and <em>Aaptos papillota</em> II (WGA-type). Type 2 includes stocks agglutinated by <em>Arachis hypogaea</em> and <em>Aaptos papillata</em> II (PNA-type). The reactions with the lectins from <em>Triticum vulgaris</em> and <em>Aaptos papillata</em> II can be correlated with the presence of N-Acetylneuraminic acid on the cell surface of these flagellates. After treatment with neuraminidase the WGA-type T. cruzi-like Morcego 1354 is agglutinated by <em>Arachis hypogaea</em> but not by the N-Acetylneuraminic acid specific lectins from <em>Triticum vulgaris</em> and <em>Aaptos papillata</em> II.</p></div>","PeriodicalId":79282,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...","volume":"256 3","pages":"Pages 342-346"},"PeriodicalIF":0.0000,"publicationDate":"1984-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-3031(84)80012-8","citationCount":"3","resultStr":"{\"title\":\"Intraspecific characterization of T. cruzi stocks by the lectins from triticum vulgaris and arachis hypogaea\",\"authors\":\"J. Schottelius , G. Uhlenbruck\",\"doi\":\"10.1016/S0174-3031(84)80012-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The agglutination behaviour of four-days-old epimastigote culture forms of <em>T. cruzi</em>, and <em>T. cruzi</em>-like stocks from different South American countries were tested with 15 lectins. Two agglutination types can be distinguished: Typ 1 includes stocks which react with <em>Triticum vulgaris</em> and <em>Aaptos papillota</em> II (WGA-type). Type 2 includes stocks agglutinated by <em>Arachis hypogaea</em> and <em>Aaptos papillata</em> II (PNA-type). The reactions with the lectins from <em>Triticum vulgaris</em> and <em>Aaptos papillata</em> II can be correlated with the presence of N-Acetylneuraminic acid on the cell surface of these flagellates. After treatment with neuraminidase the WGA-type T. cruzi-like Morcego 1354 is agglutinated by <em>Arachis hypogaea</em> but not by the N-Acetylneuraminic acid specific lectins from <em>Triticum vulgaris</em> and <em>Aaptos papillata</em> II.</p></div>\",\"PeriodicalId\":79282,\"journal\":{\"name\":\"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...\",\"volume\":\"256 3\",\"pages\":\"Pages 342-346\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0174-3031(84)80012-8\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0174303184800128\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0174303184800128","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
用15种凝集素对产自南美不同国家的克氏T. (T. cruzi)和克氏T. (T. cruzi)样砧木的4日龄拟马毛囊培养体的凝集行为进行了试验。可区分两种凝集类型:1型包括与普通小麦和小麦II型(wga型)反应的原料。2型包括由arachhis hypogaea和Aaptos papillata II (pna型)凝集的砧木。这些鞭毛虫与普通小麦和乳突Aaptos papillata II的凝集素的反应可能与这些鞭毛虫细胞表面n -乙酰神经氨酸的存在有关。经神经氨酸酶处理后，wga型T. cruzi-like Morcego 1354可被花生凝集，但不能被普通小麦和乳突Aaptos papillata II的n -乙酰神经氨酸特异性凝集。
Intraspecific characterization of T. cruzi stocks by the lectins from triticum vulgaris and arachis hypogaea
The agglutination behaviour of four-days-old epimastigote culture forms of T. cruzi, and T. cruzi-like stocks from different South American countries were tested with 15 lectins. Two agglutination types can be distinguished: Typ 1 includes stocks which react with Triticum vulgaris and Aaptos papillota II (WGA-type). Type 2 includes stocks agglutinated by Arachis hypogaea and Aaptos papillata II (PNA-type). The reactions with the lectins from Triticum vulgaris and Aaptos papillata II can be correlated with the presence of N-Acetylneuraminic acid on the cell surface of these flagellates. After treatment with neuraminidase the WGA-type T. cruzi-like Morcego 1354 is agglutinated by Arachis hypogaea but not by the N-Acetylneuraminic acid specific lectins from Triticum vulgaris and Aaptos papillata II.
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