补骨精水提物的乙酸乙酯部分不表现出急性或亚慢性毒性。Wistar大鼠的实验研究

F. Agbogba, M. Sènou, Ap Tchogou, J. Lokonon, Ti Sacramento, E. Mèdoatinsa, Re Kanfon, E. Atakpa, T. Dougnon, D. Agbangnan, F. Loko, A. Laleye, J. Ategbo, S. Baba-Moussa, A. Agbonon, A. Sezan
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引用次数: 4

摘要

补骨孢子囊是一种热带植物,其根皮用于治疗贫血。本工作旨在评价该树皮水提物的乙酸乙酯部分的安全性。方法:按照OECD推荐的方法,将提取液的乙酸乙酯部分按2000 mg / Kg体重单次给予Wistar大鼠进行急性口服毒性试验,或按200 mg / Kg体重每日给予28天进行亚慢性口服毒性试验。在第0天,急性期第14天,亚慢性期第28天,对大鼠称重并采集血液进行检测。肝功能检查时测定转氨酶AST和ALT的活性,肾功能检查时测定血尿素和肌酐,免疫平衡时测定血白细胞。这些分析是由肝脏、肾脏和免疫器官脾脏的组织学补充的。结果:在急性和亚慢性口服毒性试验中,大鼠的体重、肝、肾和免疫平衡以及这些器官的组织学均未受到影响,提示提取物部位的安全性。结论:补骨牛皮根皮水提物乙酸乙酯部分无急性或亚慢性口服毒性。这种效果可能与其富含具有细胞保护作用的黄酮类化合物有关。生物耐受性的研究值得通过慢性毒性试验和适当的临床试验继续进行。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ethyl acetate fraction of Psorospermum febrifugum Spach aqueous extract did not exhibit acute or sub-chronic toxicity. Experimental study on Wistar rats
Psorospermum febrifugum Spach (Clusiaceae) was a tropical plant whose root bark was used to treat anemia. This work aimed to evaluate the safety of the ethyl acetate fraction of the aqueous extract of this bark. Methods: The ethyl acetate fraction of the extract was administered to Wistar rats in a single dose of 2000 mg / Kg body weight for acute oral toxicity test or daily doses of 200 mg / Kg of body weight during 28 days for sub-chronic oral toxicity test, as recommended by the OECD. At day 0, then at day 14 for the acute phase and day 28 for the sub-chronic phase, the rats were weighed and their blood collected for tests. The activity of transaminases AST and ALT were measured in the liver function tests, blood urea and creatinine were measured for renal function tests and blood leukocytes were counted for the immune balance. These analyzes were supplemented by the histology of the liver, kidneys and spleen, an immune organ. Results: In acute and sub-chronic oral toxicity tests, rat’s weight, liver, kidney and immune balances as well as these organs histology were not affected, suggesting the safety of the extract fraction. Conclusion: The ethyl acetate fraction of the aqueous extract of the root bark of Psorospermum febrifugum did not reveal any acute or sub-chronic oral toxicity. This effect could be related to its richness in flavonoids which have cytoprotective effects. The study of biological tolerance deserves to be continued by the chronic toxicity test and appropriate clinical trials.
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