两种罗非鱼(nilochromis和Sarthrodon Galilaeus)随机扩增多态性DNA的遗传多样性

The Open Biology Journal Pub Date : 2020-09-25 DOI:10.2174/1874196702008010022

O. M. Omer, A. H. Abdalla, Z. Mahmoud

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引用次数: 1

摘要

从代表白尼罗河、青尼罗河和尼罗河的八个地点收集了鱼类标本。在使用8个引物进行RAPD-PCR分子分析之前，从单个标本中取出鳃和背鳍组织样本，分别在无水乙醇中保存。使用OPA-04、OPA-13、OPA-03、OPA-06、OPA-07、OPA-09、OPA-10和RAPD-8进行DNA分析，得到了不同的条带。

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Genetic Diversity of Two Tilapia Species (Oreochromis Niloticus and Sarthrodon Galilaeus) Using Random Amplified Polymorphic DNA

Fish specimens were collected from eight sites representing the White Nile, Blue Nile and the River Nile. Tissue samples from gills and dorsal fin were removed from individual specimens and preserved separately in absolute ethanol prior to molecular analysis by RAPD-PCR using eight primers. DNA analysis using OPA-04, OPA-13, OPA-03, OPA-06, OPA-07, OPA-09, OPA-10 and RAPD-8 produced different bands for each.

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The Open Biology Journal

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