代谢性谷氨酸受体(mGluR)亚型5药理学特征的原代肝细胞分离优化:还原研究

Annual ORNL Biomedical Science and Engineering Center Conference. ORNL Biomedical Science and Engineering Center Conference Pub Date : 2021-09-29 DOI:10.4081/bse.2021.151

C. Berardo, A. Ferrigno, Veronica Siciliano, M. Cagna, P. Richelmi, M. Vairetti, L. G. Pasqua

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引用次数: 0

摘要

为了尽量减少实验中使用的动物数量，根据最终目标选择合适的酶是很重要的。在我们的工作中，我们证明了胶原酶IV在维持大鼠肝细胞功能性跨膜受体方面的优势，从而证明了其药理活性。

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Optimization of primary hepatocyte isolation for the pharmacological characterization of metabotropic glutamate receptor (mGluR) s ubtype 5: A study on Reduction

To minimize the number of animals used during experiments, it is important to choose the suitable enzyme according to the final goal. In our work, we demonstrated the superiority of collagenase IV in the maintenance of functional transmembrane receptor, thus the pharmacological activity, in isolated rat hepatocytes.

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Annual ORNL Biomedical Science and Engineering Center Conference. ORNL Biomedical Science and Engineering Center Conference

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