香茅提取物对瑞士白化大鼠和志愿者按蚊的驱避作用

O. Oluyemi, Adewumi Tunde Ayodele, Ajayi Kehinde Oluyemi
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引用次数: 3

摘要

昆虫传播的疾病仍然是全世界疾病和死亡的主要来源。斯蒂芬按蚊和库氏按蚊是疟疾的重要传播媒介,疟疾仍然是热带世界的一个主要公共卫生问题。本研究以瑞士白化大鼠和人体为实验对象,研究柠檬草提取物对按蚊的驱避作用。采用甲醇、氯仿和水提取植物叶片的生物活性成分,测定植物提取物的定性和定量成分,采用25只实验室饲养的饥饿5小时的3 d龄的蚊,提取液和市售杀虫剂N,将n -二乙基-3-甲基苯甲胺(DEET)局部涂于人类志愿者的手部裸露部分皮肤和瑞士白化大鼠腹部,剃除腹部毛后,观察蚊子的着陆率,计算驱避率。结果表明,甲醇提取物得率最高,达10.3%,各提取物均含有单宁和生物碱。避蚊胺在人类志愿者和大鼠身上的驱蚊率均为100%,使用300分钟后,在人类志愿者身上,水、氯仿和甲醇提取物,在使用60分钟后,驱蚊率从100%降至94%,在使用120分钟后,驱蚊率从100%降至94%,在使用150分钟后,驱蚊率从100%降至83%,而在瑞士白化大鼠身上,水、氯仿和甲醇提取物,在使用90分钟后,驱蚊率从100%降至87%。150分钟后100% ~ 87%,180分钟后100% ~ 90%。甲醇提取物。柑桔叶可用于研制局部驱蚊膏,有效防治疟疾。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Repellence Activity of Cymbopogon Citratus (DC) Extracts on Anopheles Mosquitoes using Swiss Albino Rat and Human Volunteer
Insect-transmitted disease remains a major source of illness and death worldwide.Anopheles stephensiandAnopheles culicifaciesare the important vectors of malaria, malaria continues to be a major public health problem in the tropical world. This study is aimed at carrying out repellence activity ofCymbopogon citratus(Lemon grass) extracts onAnophelesmosquitoes using swiss albino rat and human volunteers.Methanol, chloroform and water were used to extract the bioactive compounds of plant leaves, qualitative and quantitative phytochemical components of plant extracts were determined, twenty-five laboratory-reared 3 day oldAnophelesmosquitoes which had been starved for 5 hours were used, extracts and the commercial insecticide N, N-diethyl-3-methylbenzamide (DEET) were applied topically on the skin of uncovered portions of the hand of the human volunteers and abdomen of swiss albino rat after the abdominal hairs has been shaved and the landing rate of mosquitoes were observed to calculate percentage repellency.The result revealed that methanol extract had the highest percentage yield of 10.3%, tannin and alkaloid were present in all extracts. DEET had 100% repellency toAnophelesmosquitoes on both human volunteers and rats for 300 minutes post application, in human volunteers, water, chloroform and methanol extracts, has percentage repellency reduced from 100% to 94% after 60 minutes, 100% to 94% after 120 minutes and 100% to 83% after 150 minutes post application respectively while in swiss albino rat, water, chloroform and methanol extracts, percentage repellency reduce from 100% to 87% after 90 minutes, 100% to 87% 150 minutes and 100% to 90% after 180 minutes post application respectively.Methanol extracts ofC. citratusleaves could be used for the development of topical cream that repels mosquitoes for effective control of malaria.
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