O. Boychuk, K. Pershina, E. Kolomiets, Yelyzaveta Pletenets
{"title":"氧化铁存在下,酶制剂莴苣与天然层状二氧化铝的结合特性","authors":"O. Boychuk, K. Pershina, E. Kolomiets, Yelyzaveta Pletenets","doi":"10.33609/2708-129x.89.04.2023.90-101","DOIUrl":null,"url":null,"abstract":"IR and X-ray analyses of bentonite modified with magnetite and enzyme established the dependence of enzyme immobilization on the presence of iron oxides. IR studies have proven that the binding of the enzyme to the carrier is adsorption in nature without stable chemical interactions. This type of binding does not significantly affect the change in the structure of the enzyme module. Characteristic bands of vibrations of amide groups of proteins were detected at 1637 cm-1 ( ν-vibrations of C=O bonds (amide I)), 713 cm-1 (δ – vibrations of O=C-N in the plane (amide IV)), which can participate in the sorption binding of the enzyme to bentonite and the SiO2 group, which is characterized by a weak connection of the outer part of protein with the formation of a surface structure, which disappears over time due to the diffusion of molecules into the pores of the mineral. Comparison of the basal distances in the samples established the following dependence: 12.43 Å bentonite+ Fe3O4+enz (48 h) > 12.38; Å natural bentonite> 12.31; Å bentonite+ Fe3O4+enz (2 hours) > 11.91; Å bentonite+ Fe3O4 , which indicates a high probability of iron compounds entering the interlayer space due to the hydrolysis of magnetite with the formation of Fe-(OH)n bonds and unstable compounds. According to the research results, a scheme of the interaction of the enzyme with modified layered iron aluminosilicate is proposed: 1 – transport of the enzyme to the surface (phase distribution boundaries); 2 – deformation of the enzyme under the action of the surface as a result of a polycondensation reaction with the formation of water molecules; 3 – entry of water molecules into the interlayer space; 4 – hydrolysis of magnetite particles; 5 – extraction of iron hydroxide from the interlayer space due to the formation of aggregates with an enzyme molecule that has a related adsorption center.","PeriodicalId":23394,"journal":{"name":"Ukrainian Chemistry Journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. 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Characteristic bands of vibrations of amide groups of proteins were detected at 1637 cm-1 ( ν-vibrations of C=O bonds (amide I)), 713 cm-1 (δ – vibrations of O=C-N in the plane (amide IV)), which can participate in the sorption binding of the enzyme to bentonite and the SiO2 group, which is characterized by a weak connection of the outer part of protein with the formation of a surface structure, which disappears over time due to the diffusion of molecules into the pores of the mineral. Comparison of the basal distances in the samples established the following dependence: 12.43 Å bentonite+ Fe3O4+enz (48 h) > 12.38; Å natural bentonite> 12.31; Å bentonite+ Fe3O4+enz (2 hours) > 11.91; Å bentonite+ Fe3O4 , which indicates a high probability of iron compounds entering the interlayer space due to the hydrolysis of magnetite with the formation of Fe-(OH)n bonds and unstable compounds. According to the research results, a scheme of the interaction of the enzyme with modified layered iron aluminosilicate is proposed: 1 – transport of the enzyme to the surface (phase distribution boundaries); 2 – deformation of the enzyme under the action of the surface as a result of a polycondensation reaction with the formation of water molecules; 3 – entry of water molecules into the interlayer space; 4 – hydrolysis of magnetite particles; 5 – extraction of iron hydroxide from the interlayer space due to the formation of aggregates with an enzyme molecule that has a related adsorption center.\",\"PeriodicalId\":23394,\"journal\":{\"name\":\"Ukrainian Chemistry Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ukrainian Chemistry Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33609/2708-129x.89.04.2023.90-101\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ukrainian Chemistry Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33609/2708-129x.89.04.2023.90-101","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
对氧化铁改性膨润土的红外和x射线分析表明,氧化铁的存在对酶的固定化具有依赖性。红外研究证明,酶与载体的结合在本质上是吸附的,没有稳定的化学相互作用。这种类型的结合不会显著影响酶模块结构的变化。Characte-ristic频段的振动酰胺组蛋白的检测在1637 cm - 1(ν—C = O债券(我)酰胺),713 cm - 1(δ- O =碳氮的振动平面(酰胺(四)),它可以参与酶的吸附结合膨润土和二氧化硅集团的特点是弱连接外部的蛋白质与表面结构的形成,随着时间的推移而消失,由于分子扩散进入毛孔的矿物。对比样品中的基距,建立了以下关系:12.43 Å膨润土+ Fe3O4+enz (48 h) > 12.38;Å天然膨润土- the > 12.31;Å膨润土+ Fe3O4+enz(2小时)> 11.91;Å膨润土+ Fe3O4,表明由于磁铁矿水解形成Fe-(OH)n键和不稳定化合物,铁化合物极有可能进入层间空间。根据研究结果,提出了一种酶与改性层状铝硅酸铁相互作用的方案:1 -酶向表面运输(相分布边界);2 -酶在表面作用下发生变形,产生缩聚反应,形成水分子;3 -水分子进入层间空间;4 -磁铁矿颗粒的水解;5 -由于与具有相关吸附中心的酶分子形成聚集体,从层间空间中提取氢氧化铁。
CHARACTERISTICS OF THE BINDING BETWEEN THE ENZYME PREPARATION RAPHANUS SATIVUS L. AND NATURAL LAYERED ALUMOSILICA IN THE PRESENCE OF IRON OXIDES
IR and X-ray analyses of bentonite modified with magnetite and enzyme established the dependence of enzyme immobilization on the presence of iron oxides. IR studies have proven that the binding of the enzyme to the carrier is adsorption in nature without stable chemical interactions. This type of binding does not significantly affect the change in the structure of the enzyme module. Characteristic bands of vibrations of amide groups of proteins were detected at 1637 cm-1 ( ν-vibrations of C=O bonds (amide I)), 713 cm-1 (δ – vibrations of O=C-N in the plane (amide IV)), which can participate in the sorption binding of the enzyme to bentonite and the SiO2 group, which is characterized by a weak connection of the outer part of protein with the formation of a surface structure, which disappears over time due to the diffusion of molecules into the pores of the mineral. Comparison of the basal distances in the samples established the following dependence: 12.43 Å bentonite+ Fe3O4+enz (48 h) > 12.38; Å natural bentonite> 12.31; Å bentonite+ Fe3O4+enz (2 hours) > 11.91; Å bentonite+ Fe3O4 , which indicates a high probability of iron compounds entering the interlayer space due to the hydrolysis of magnetite with the formation of Fe-(OH)n bonds and unstable compounds. According to the research results, a scheme of the interaction of the enzyme with modified layered iron aluminosilicate is proposed: 1 – transport of the enzyme to the surface (phase distribution boundaries); 2 – deformation of the enzyme under the action of the surface as a result of a polycondensation reaction with the formation of water molecules; 3 – entry of water molecules into the interlayer space; 4 – hydrolysis of magnetite particles; 5 – extraction of iron hydroxide from the interlayer space due to the formation of aggregates with an enzyme molecule that has a related adsorption center.