利用线粒体DNA标记测定爱尔兰选择的亲母猪品种的遗传结构

Yelyzaveta Budakva
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引用次数: 2

摘要

传统上,线粒体基因组被描述为一个“分子钟”,用于跟踪沿着母系的系统发育历史。特别关注来自乌克兰波尔塔瓦地区Globyno镇RPE“Globinsky猪场”的商品猪(大白×长白)× Maxgro的线粒体DNA单倍型分布。为了研究猪的线粒体DNA杂交标记的遗传结构,使用了母系遗传。DNA标记是调查亲母猪品种起源的方便工具。应用多重分析PCR-RFLP(聚合酶链反应-限制性片段长度多态性)检测杂交猪线粒体基因组15558-15917位点之间的d环可变区域,可以确定实验样品的亲母单倍型(n=20)。因此,根据Pochernyaev K. F.开发的多位点系统,测定猪的线粒体单倍型(用拉丁字母从A到P表示)可以确定猪实验样品(n=20)的真正亲母单倍型,正如上述条款中Tas I网站的存在所证明的那样,它实际上决定了线粒体DNA的单倍型。根据研究结果确定了不同品种的单倍型特征,即4只动物具有单倍型C -长白猪(乌克兰、波兰)。6头猪的线粒体单倍型为N -大白(亚洲型),7头猪的线粒体单倍型为O -长白猪,1头猪的线粒体单倍型为G -野猪和跨界品种威尔士(意大利)。2个单倍型的代表-在家猪品种中没有发现。根据杂交猪已建立的亲母单倍型,携带O型单倍型的动物是瑞典和爱尔兰子宫群中使用的斯堪的纳维亚母猪F1的代表,Maxgro终亲本系参与杂交系统。鉴定出的线粒体单倍型被发现是爱尔兰杂交猪的品种特异性,这被线粒体基因组的多态性所证实,即使在复杂的杂交方案中,线粒体基因组也是一个客观的标记。这项工作是在乌克兰国家农业科学院31.01.00.07.F的支持下完成的。“研究SNP在标记相关猪育种中使用的多效基因”。诺博士。0121 u109838。以Pochernyaev k.f.对限制性片段组合的系统化研究为例,我建议建立一个猪最终杂交的线粒体DNA参考单倍型数据库。未来,将在进一步的研究中用于重建跨界品种商品猪的人口统计学历史。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of the genetic structure of pro-maternal pig breeds of Irish selection using mitochondrial DNA markers
Traditionally, the mitochondrial genome is characterized as a “molecular clock” for tracking the history of phylogeny along the maternal line. Particular attention is paid to the distribution of mitochondrial DNA haplotypes among commercial pigs (Large White × Landrace) × Maxgro from RPE “Globinsky Pig Farm”, Globyno town, Poltava region, Ukraine. For the study of the genetic structure of the pigs’ hybrid markers of mitochondrial DNA — a maternal type of inheritance was used. DNA markers are a convenient tool for investigating the origin of pro-maternal pig breeds. Application of multiplex analysis PCR-RFLP (Polymerase chain reaction-restriction fragment length polymorphism) when examining the variable area of the D-loop between sites 15558–15917 mitochondrial genome of hybrid pigs made it possible to determine the pro-maternal haplotypes of the experimental sample (n=20). Thus, according to the multisite system developed by Pochernyaev K. F., determination of mitochondrial haplotypes of pigs, which are denoted by Latin letters from A to P allowed to determine the true pro-maternal haplotypes of the experimental sample of pigs (n=20), as evidenced by the presence of the Tas I website in the above-mentioned provisions what actually determine the haplotypes of mitochondrial DNA. According to the results of the study defined haplotypes characterize different breeds, namely 4 animals with haplotype C — Landrace (Ukraine, Poland). 6 pigs have mitochondrial haplotype N — Large White (Asian type) and 7 pigs with mitochondrial haplotype O — Landrace. 1 animal with haplotype G — wild pig and cross-border breed Wales (Italy). 2 representatives of haplotype D — not found among the breeds of domestic pigs. According to the established pro-maternal haplotypes of hybrid pigs, animals-carriers of haplotype O are representatives of Scandinavian female pigs F1 as used in uterine herds in Sweden and Ireland with the participation of the Maxgro terminal parent line in the hybridization system. Identified mitochondrial haplotypes were found to be breed-specific to hybrid pigs of Irish breeding, this is confirmed by the established polymorphism of the mitochondrial genome which is an objective marker even in complex hybridization schemes. The work was done with the support of the National Academy of Agrarian Sciences of Ukraine 31.01.00.07.F. “Investigate the pleiotropic effect gens that the SNP use in marker-associated pig breeding”. DR no. 0121U109838. Following the example of the developed systematization of the combination of restricted fragments by Pochernyaev K. F. in the future, I propose to create a database of reference haplotypes of mitochondrial DNA of pigs’ final hybrid. In the future, it will be used in further research to reconstruct the demographic history of commercial pigs of cross-border breeds.
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